Elderly dendritic cells respond to LPS/IFN-Î³ and CD40L stimulation despite incomplete maturation
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There is evidence that dendritic cells (DCs) undergo age-related changes that modulate their function with their key role being priming antigen-specific effector T cells. This occurs once DCs develop into antigen-presenting cells in response to stimuli/danger signals. However, the effects of aging on DC responses to bacterial lipopolysaccharide (LPS), the proinflammatory cytokine interferon (IFN)-Î³ and CD40 ligand (CD40L) have not yet been systematically evaluated. We examined responses of blood myeloid (m)DC1s, mDC2s, plasmacytoid (p)DCs, and monocyte-derived DCs (MoDCs) from young (21â€“40 years) and elderly (60â€“84 years) healthy human volunteers to LPS/IFN-Î³ or CD40L stimulation. All elderly DC subsets demonstrated comparable up-regulation of co-stimulatory molecules (CD40, CD80 and/or CD86), intracellular pro-inflammatory cytokine levels (IFN-Î³, tumour necrosis factor (TNF)-Î±, IL-6 and/or IL-12), and/or secreted cytokine levels (IFN-Î±, IFN-Î³, TNF-Î±, and IL-12) to their younger counterparts. Furthermore, elderly-derived LPS/IFN-Î³ or CD40L-activated MoDCs induced similar or increased levels of CD8 + and CD4 + T cell proliferation, and similar T cell functional phenotypes, to their younger counterparts. However, elderly LPS/IFN-Î³-activated MoDCs were unreliable in their ability to up-regulate chemokine (IL-8 and monocyte chemoattractant protein (MCP)-1) and IL-6 secretion, implying an inability to dependably induce an inflammatory response. A key age-related difference was that, unlike young-derived MoDCs that completely lost their ability to process antigen, elderly-derived MoDCs maintained their antigen processing ability after LPS/IFN-Î³ maturation, measured using the DQ-ovalbumin assay; this response implies incomplete maturation that may enable elderly DCs to continuously present antigen. These differences may impact on the efficacy of anti-pathogen and anti-tumour immune responses in the elderly.
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