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dc.contributor.authorAcquah, C.
dc.contributor.authorDanquah, M.
dc.contributor.authorChan, Y.
dc.contributor.authorLoo Chin Moy, Charles
dc.contributor.authorOngkudon, C.
dc.contributor.authorLau, John
dc.date.accessioned2018-05-18T07:56:20Z
dc.date.available2018-05-18T07:56:20Z
dc.date.created2018-05-18T00:23:00Z
dc.date.issued2018
dc.identifier.citationAcquah, C. and Danquah, M. and Chan, Y. and Loo Chin Moy, C. and Ongkudon, C. and Lau, J. 2018. Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation. Separation Science and Technology. 53 (13): pp. 2100-2111.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/66847
dc.identifier.doi10.1080/01496395.2018.1443139
dc.description.abstract

The introduction of aptameric ligands onto disk-monolithic adsorbent, representing a unique strategy for convective isolation of target molecules with high specificity and selectivity, is investigated for the first time. Experimental results showed that the disk monolith possessed a good permeability of 1.67 ± 0.05 × 10 –14  m 2 (RSD = 3.2%). The aptameric ligand density for the aptamer-modified disk monolith was 480 pmol/uL. Chromatographic analysis of the aptamer disk-monolith efficiency showed an optimum linear velocity of 126 cm/min (˜0.25 mL/min) at room temperatures 25 ± 2°C. The theoretical number of plates corresponding to the optimum linear velocity was 128.2 with an height equivalent to the theoretical plate of 0.022 mm. The disk aptamer-immobilised monolithic system demonstrated good selectivity and isolation of thrombin from non-targets.

dc.publisherTaylor & Francis Inc.
dc.titleChromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation
dc.typeJournal Article
dcterms.source.startPage1
dcterms.source.endPage12
dcterms.source.issn0149-6395
dcterms.source.titleSeparation Science and Technology (Philadelphia)
curtin.departmentCurtin Malaysia
curtin.accessStatusFulltext not available


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