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dc.contributor.authorArifin, Yalun
dc.contributor.authorSabri, S.
dc.contributor.authorSugiarto, H.
dc.contributor.authorKrömer, J.
dc.contributor.authorVickers, C.
dc.contributor.authorNielsen, L.
dc.identifier.citationArifin, Y. and Sabri, S. and Sugiarto, H. and Krömer, J. and Vickers, C. and Nielsen, L. 2010. Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture. Journal of Biotechnology. 156 (4): pp. 275-278.

Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial Escherichia coli strains are unable to utilize sucrose. E. coli W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesirable in fed-batch conditions where sugar levels are low between feeding pulses. Sucrose uptake rates were improved by removal of the cscR gene, which encodes a protein that represses expression of the sucrose utilization genes at low sucrose concentrations. Poly-3-hydroxybutyrate (PHB) was used as a model compound in order to assess the effect of improved sugar utilization on bio-production. In the cscR knockout strain, production from sucrose was improved by 50%; this strain also produced 30% more PHB than the wild-type using glucose. This result demonstrates the feasibility of utilizing sucrose as an industrial feedstock for E. coli-based bioprocesses in high cell density culture. © 2011.

dc.publisherElsevier Science
dc.titleDeletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
dc.typeJournal Article
dcterms.source.titleJournal of Biotechnology
curtin.departmentCurtin Sarawak
curtin.accessStatusFulltext not available

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