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dc.contributor.authorHuda, N.
dc.contributor.authorGauri, B.
dc.contributor.authorBenson, Heather
dc.contributor.authorChen, Y.
dc.date.accessioned2018-05-18T07:59:39Z
dc.date.available2018-05-18T07:59:39Z
dc.date.created2018-05-18T00:22:54Z
dc.date.issued2018
dc.identifier.citationHuda, N. and Gauri, B. and Benson, H. and Chen, Y. 2018. A Stability Indicating HPLC Assay Method for Analysis of Rivastigmine Hydrogen Tartrate in Dual-Ligand Nanoparticle Formulation Matrices and Cell Transport Medium. Journal of Analytical Methods in Chemistry. 2018: Article ID 1841937.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/67763
dc.identifier.doi10.1155/2018/1841937
dc.description.abstract

The objective of this study was to develop and validate a method for quantitative analysis of rivastigmine hydrogen tartrate (RHT) in dual-ligand polymeric nanoparticle formulation matrices, drug release medium, and cellular transport medium. An isocratic HPLC analysis method using a reverse phase C 18 column and a simple mobile phase without buffer was developed, optimised, and fully validated. Analyses were carried out at a flow rate of 1.5 mL/min at 50°C and monitored at 214 nm. This HPLC method exhibited good linearity, accuracy, and selectivity. The recovery (accuracy) of RHT from all matrices was greater than 99.2%. The RHT peak detected in the samples of a forced degradation study, drug loading study, release study, and cellular transport study was pure and free of matrix interference. The limit of detection (LOD) and limit of quantification (LOQ) of the assay were 60 ng/mL and 201 ng/mL, respectively. The method was rugged with good intra- and interday precision. This stability indicating HPLC method was selective, accurate, and precise for analysing RHT loading and its stability in nanoparticle formulation, RHT release, and cell transport medium.

dc.publisherHindawi Publishing Corporation
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleA Stability Indicating HPLC Assay Method for Analysis of Rivastigmine Hydrogen Tartrate in Dual-Ligand Nanoparticle Formulation Matrices and Cell Transport Medium
dc.typeJournal Article
dcterms.source.volume2018
dcterms.source.issn2090-8865
dcterms.source.titleJournal of Analytical Methods in Chemistry
curtin.departmentSchool of Pharmacy and Biomedical Sciences
curtin.accessStatusOpen access


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