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dc.contributor.authorIchimizu, S.
dc.contributor.authorWatanabe, H.
dc.contributor.authorMaeda, H.
dc.contributor.authorHamasaki, K.
dc.contributor.authorNakamura, Y.
dc.contributor.authorChuang, Victor
dc.contributor.authorKinoshita, R.
dc.contributor.authorNishida, K.
dc.contributor.authorTanaka, R.
dc.contributor.authorEnoki, Y.
dc.contributor.authorIshima, Y.
dc.contributor.authorKuniyasu, A.
dc.contributor.authorKobashigawa, Y.
dc.contributor.authorMorioka, H.
dc.contributor.authorFutaki, S.
dc.contributor.authorOtagiri, M.
dc.contributor.authorMaruyama, T.
dc.identifier.citationIchimizu, S. and Watanabe, H. and Maeda, H. and Hamasaki, K. and Nakamura, Y. and Chuang, V. and Kinoshita, R. et al. 2018. Design and tuning of a cell-penetrating albumin derivative as a versatile nanovehicle for intracellular drug delivery. Journal of Controlled Release. 277: pp. 23-34.

© 2018 Human serum albumin (HSA) is a superior carrier for delivering extracellular drugs. However, the development of a cell-penetrating HSA remains a great challenge due to its low membrane permeability. We report herein on the design of a series of palmitoyl-poly-arginine peptides (CPPs) and an evaluation of their cell-penetrating effects after forming a complex with HSA for use in intracellular drug delivery. The palmitoyl CPPs forms a stable complex with HSA by anchoring itself to the high affinity palmitate binding sites of HSA. Among the CPPs evaluated, a cyclic polypeptide composed of D-dodecaarginines, palmitoyl-cyclic-(D-Arg) 12 was the most effective for facilitating the cellular uptake of HSA by HeLa cells. Such a superior cell-penetrating capability is primarily mediated by macropinocytosis. The effect of the CPP on pharmacological activity was examined using three drugs loaded in HSA via three different methods: a) an HSA-paclitaxel complex, b) an HSA-doxorubicin covalent conjugate and c) an HSA-thioredoxin fusion protein. The results showed that cell-penetrating efficiency was increased with a corresponding and significant enhancement in pharmacological activity. In conclusion, palmitoyl-cyclic-(D-Arg) 12 /HSA is a versatile cell-penetrating drug delivery system with great potential for use as a nano-carrier for a wide diversity of pharmaceutical applications.

dc.titleDesign and tuning of a cell-penetrating albumin derivative as a versatile nanovehicle for intracellular drug delivery
dc.typeJournal Article
dcterms.source.titleJournal of Controlled Release
curtin.departmentSchool of Pharmacy and Biomedical Sciences
curtin.accessStatusFulltext not available

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