Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum
MetadataShow full item record
Fusarium crown rot (FCR), an important disease of wheat and barley, is mainly caused by Fusarium graminearum, F. culmorum and F. pseudograminearum, which are also responsible for mycotoxin production. This is the first comparative investigation of their colonization on barley plants after stem base inoculation. At plant maturity, FCR symptoms were visually evaluated, fungal biomass was quantified by Real-Time quantitative PCR and deoxynivalenol (DON) was detected by enzyme-linked immunosorbent assay (ELISA). All the inoculated strains caused the typical FCR necrotic symptoms. Real-Time PCR analysis showed that F. graminearum and F. culmorum were present in the head tissues, while F. pseudograminearum colonized only up to the area including the second node of the stem. Conversely, DON was detected up to the head for all the three species. This study shows that, as already demonstrated in previous research for wheat, DON may be detected up to the head as a consequence of stem base infection by the three FCR agents.
Showing items related by title, author, creator and subject.
Fungal community, Fusarium head blight complex and secondary metabolites associated with malting barley grains harvested in Umbria, central ItalyBeccari, G.; Senatore, M.; Tini, F.; Sulyok, M.; Covarelli, Lorenzo (2018)In recent years, due to the negative impact of toxigenic mycobiota and of the accumulation of their secondary metabolites in malting barley grains, monitoring the evolution of fungal communities in a certain cultivation ...
Changes in the Fusarium head blight complex of malting barley in a three-year field experiment in ItalyBeccari, G.; Prodi, A.; Tini, F.; Bonciarelli, U.; Onofri, A.; Oueslati, S.; Limayma, M.; Covarelli, Lorenzo (2017)In this study, conducted for three years on eleven malting barley varieties cultivated in central Italy, the incidence of different mycotoxigenic fungal genera, the identification of the Fusarium species associated with ...
Effect of wheat infection timing on Fusarium head blight causal agents and secondary metabolites in grainBeccari, G.; Arellano, C.; Covarelli, Lorenzo; Tini, F.; Sulyok, M.; Cowger, C. (2019)© 2018 Fusarium head blight (FHB) results in yield loss and damaging contamination of cereal grains and can be caused by several Fusarium species. The objective of the present study was to determine, in a greenhouse ...