Dermal fibroblast extracellular matrix regulates keratinocyte behaviour
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This project explored using dermal fibroblast-derived extracellular matrices (ECMs) to recapitulate the keratinocyte microenvironmental niche in vitro. The data indicated that fibroblast-derived matrices better supported keratinocytes expansion than substrates of type I collagen. ECM composition was found to profoundly alter the gene expression profiles of keratinocytes grown on these matrices, in that foetal matrices triggered expression of cell cycle genes, whereas adult matrices triggered the expression of a balance of differentiation and cell cycle genes.
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In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like CharacteristicsWong, C.; Kinnear, B.; Sobota, R.; Ramalingam, R.; Legrand, C.; Dye, Danielle; Raghunath, M.; Lane, B.; Coombe, D. (2018)The long-term expansion of keratinocytes under serum- and feeder free conditions generally results in diminished proliferation and an increased commitment to terminal differentiation. Here we present a serum and xenogeneic ...
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Lawlor, K.; Kaur, Pritinder (2015)© 2015 by the authors; licensee MDPI, Basel, Switzerland. The human interfollicular epidermis is renewed throughout life by populations of proliferating basal keratinocytes. Though interfollicular keratinocyte stem cells ...