Direct Label-Free Imaging of Brain Tissue using Synchrotron Light: A Review of New Spectroscopic Tools for the Modern Neuroscientist
MetadataShow full item record
The incidence of brain disease and brain disorders is increasing on a global scale. Unfortunately, development of new therapeutic strategies has not increased at the same rate, and brain diseases and brain disorders now inflict substantial health and economic impacts. A greater understanding of the fundamental neurochemistry that underlies healthy brain function, and the chemical pathways that manifest in brain damage or malfunction, are required to enable and accelerate therapeutic development. A previous limitation to the study of brain function and malfunction has been the limited number of techniques that provide both a wealth of biochemical information, and spatially resolved information (i.e., there was a previous lack of techniques that provided direct biochemical or elemental imaging at the cellular level). In recent times, a suite of direct spectroscopic imaging techniques, such as Fourier transform infrared spectroscopy (FTIR), X-ray fluorescence microscopy (XFM), and X-ray absorption spectroscopy (XAS) have been adapted, optimized and integrated into the field of neuroscience, to fill the above mentioned capability-gap. Advancements at synchrotron light sources, such as improved light intensity/flux, increased detector sensitivities and new capabilities of imaging/optics, has pushed the above suite of techniques beyond “proof-of-concept” studies, to routine application to study complex research problems in the field of neuroscience (and other scientific disciplines). This review examines several of the major advancements that have occurred over the last several years, with respect to FTIR, XFM and XAS capabilities at synchrotron facilities, and how the increases in technical capabilities have being integrated and used in the field of neuroscience.
Showing items related by title, author, creator and subject.
Manuel, Christopher D. (2002)The goal of oil and gas exploration using seismic methods is to accurately locate geological structures that could host such reserves. As the search for these resources tends towards more complex regions, it is necessary ...
The need for improved brain lesion segmentation techniques for children with cerebral palsy: A reviewPagnozzi, A.; Gal, Y.; Boyd, Roslyn; Fiori, S.; Fripp, J.; Rose, S.; Dowson, N. (2015)© 2015 Elsevier Ltd. Cerebral palsy (CP) describes a group of permanent disorders of posture and movement caused by disturbances in the developing brain. Accurate diagnosis and prognosis, in terms of motor type and severity, ...
Novel bio-spectroscopic imaging reveals disturbed protein homeostasis and thiol redox with protein aggregation prior to hippocampal CA1 pyramidal neuron death induced by global brain ischemia in the ratHackett, Mark; Smith, S.; Caine, S.; Nichol, H.; George, G.; Pickering, I.; Paterson, P. (2015)Global brain ischemia resulting from cardiac arrest and cardiac surgery can lead to permanent brain damage and mental impairment. A clinical hallmark of global brain ischemia is delayed neurodegeneration, particularly ...