Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition
MetadataShow full item record
© 2017 Taylor & Francis. Diet profoundly influences the behavior of animals across many phyla. Despite this, most laboratories using model organisms, such as Drosophila, use multiple, different, commercial or custom-made media for rearing their animals. In addition to measuring growth, fecundity and longevity, we used several behavioral and physiological assays to determine if and how altering food media influence wild-type (Canton S) Drosophila melanogaster, at larval, pupal, and adult stages. Comparing 2 commonly used commercial food media we observed several key developmental and morphological differences. Third-instar larvae and pupae developmental timing, body weight and size, and even lifespan significantly differed between the 2 diets, and some of these differences persisted into adulthood. Diet was also found to produce significantly different thermal preference, locomotory capacity for geotaxis, feeding rates, and lower muscle response to hormonal stimulation. There were no differences, however, in adult thermal preferences, in the number or viability of eggs laid, or in olfactory learning and memory between the diets. We characterized the composition of the 2 diets and found particularly significant differences in cholesterol and (phospho)lipids between them. Notably, diacylglycerol (DAG) concentrations vary substantially between the 2 diets, and may contribute to key phenotypic differences, including lifespan. Overall, the data confirm that 2 different diets can profoundly influence the behavior, physiology, morphology and development of wild-type Drosophila, with greater behavioral and physiologic differences occurring during the larval stages.
Showing items related by title, author, creator and subject.
An investigation of the physiological and biochemical responses elicited by Panulirus cygnus to harvesting, holding and live transport.Spanoghe, Patrick T. (1996)The western rock lobster (WRL), Panulirus cygnus is a decapod crustacean which is found in abundance in the coastal waters of Western Australia and which supports a major fishery of economic importance for the State, with ...
The effects of dietary organic selenium supplementation on physiological status of cultured marron, Cherax cainii (Austin, 2002)Nugroho, Rudy Agung (2014)Six 90-day feeding experiments including outdoor commercial marron ponds trial were designed and conducted to investigate the effects of dietary Sel-Plex® as a source of organic selenium (OS) supplementation in marron, ...
Effects of organic selenium supplementation on growth, glutathione peroxidase activity and histopathology in juvenile barramundi (Lates calcarifer Bloch 1970) fed high lupin meal-based dietsIlham; Fotedar, Ravi; Munilkumar, S. (2016)Very limited information is available on the relationship between dietary selenium (Se) and plant protein (PP) sources in carnivorous marine aquaculture species. Therefore, this study employed a 2 × 3 experimental layout ...