Chloride ion tolerance and pyrite bioleaching capabilities of pure and mixed halotolerant, acidophilic iron- and sulfur-oxidizing cultures
MetadataShow full item record
© 2018 Elsevier Ltd The search for halotolerant acidophilic microorganisms to increase the efficiency of bioleaching processes in regions of fresh water scarcity has been ongoing for the past two decades. In this study, three pure cultures (V6, V8 and M8) and four enrichment cultures (14C, L2-21, L4-9 and L6-11) from low pH, high saline environments were characterized for their ability to oxidize soluble iron and inorganic sulfur in the presence of increasing concentrations of chloride ion. The mixed cultures 14C and L2-21 contained predominantly Acidihalobacter and Acidithiobacillus spp., respectively, while L4-9 and L6-11 predominantly contained Ferroplasma spp. Cultures V6, V8, 14C and L2-21 were assessed for their ability to oxidize 1% pyrite at 9, 15 and 30 g/L chloride ion. Results showed that pure cultures V6 and V8 and mixed culture 14C were able to oxidize pyrite at chloride ion concentrations of 30 g/L, which is higher than the chloride concentration found in seawater (19 g/L). L2-21 was unable to oxidise pyrite, possibly due to the predominant presence of sulfur oxidizing microorganisms in the mixed culture. This illustrates the potential applicability of the cultures for saline water bioleaching and biooxidation of sulfide ores.
Showing items related by title, author, creator and subject.
Bioleaching in brackish waters—effect of chloride ions on the acidophile population and proteomes of model speciesZammit, Carla; Mangold, S.; rao Jonna, V.; Mutch, Lesley; Watling, Helen; Dopson, M.; Watkin, Elizabeth (2012)High concentrations of chloride ions inhibit the growth of acidophilic microorganisms used in biomining, a problem particularly relevant to Western Australian and Chilean biomining operations. Despite this, little is known ...
The mineralogy and processing potential of the Commonwealth project in the Molong Volcanic Belt, central eastern New South Wales, AustraliaAylmore, Mark; Eksteen, Jacques; Jones, M.G.; Wells, M. (2019)The Commonwealth prospect area comprises the base metal-gold Commonwealth Mine, Commonwealth South gold deposit and the Silica Hill deposit located 100 km north of Orange in New South Wales, Australia. Impact Minerals ...
Gold accumulation in the archaean witwatersrand basin, south africa-evidence from concentrically laminated pyriteAgangi, Andrea; Hofmann, A.; Rollion-Bard, C.; Marin-Carbonne, J.; Cavalazzi, B.; Large, R.; Meffre, S. (2015)Concentrically laminated pyrite is a relatively common, although volumetrically minor, component of auriferous conglomerates in the Archaean (ca. 3.0-2.7Ga) Witwatersrand Basin of South Africa. This type of pyrite contains ...