Distinct inflammatory responses differentiate cerebral infarct from transient ischaemic attack
|dc.identifier.citation||Armstrong, C. and Bosio, E. and Neil, C. and Brown, S. and Hankey, G. and Fatovich, D. 2017. Distinct inflammatory responses differentiate cerebral infarct from transient ischaemic attack. Journal of Clinical Neuroscience. 35: pp. 97-103.|
We previously reported on a 26-year-old patient who presented early during a large and eventually fatal cerebral infarct. Microarray analysis of blood samples from this patient demonstrated initially up-regulated and subsequently down-regulated Granzyme B (GzmB) expression, along with progressive up-regulation of genes for S100 calcium binding protein A12 (S100A12) and matrix metalloproteinase 9 (MMP-9). To confirm these findings, we investigated these parameters in patients with suspected stroke presenting within 6 h of symptom onset to a single centre. Blood samples were taken at enrolment, then 1 h, 3 h and 24 h post-enrolment for the examination of cellular, protein and genetic changes. Patients with subsequently confirmed ischaemic (n = 18) or haemorrhagic stroke (n = 11) showed increased intracellular concentrations of GzmB in all cell populations investigated (CD8+, CD8-and Natural Killer [NK] cells). Infarct patients, however, demonstrated significantly reduced GzmB gene expression and increased circulating MMP-9 and S100A12 levels in contrast to transient ischaemic attack (TIA) patients or healthy controls. Furthermore, a pronounced neutrophilia was noted in the infarct and haemorrhage groups, while TIA patients (n = 9) reflected healthy controls (n = 10). These findings suggest a spectrum of immune response during stroke. TIA showed few immunological changes in comparison to infarct and haemorrhage, which demonstrated inhibition of GzmB production and a rise in neutrophil numbers and neutrophil-associated mediators. This implies a greater role of the innate immune system. These markers may provide novel targets for inhibition and reduction of secondary injury.
|dc.title||Distinct inflammatory responses differentiate cerebral infarct from transient ischaemic attack|
|dcterms.source.title||Journal of Clinical Neuroscience|
|curtin.department||School of Nursing, Midwifery and Paramedicine|
|curtin.accessStatus||Fulltext not available|
Files in this item
There are no files associated with this item.