Microfluidic chip-based one-step fabrication of an artificial photosystem i for photocatalytic cofactor regeneration
MetadataShow full item record
We propose herein, a one-step method to assemble the immobilized artificial photosystem I (IAPSI) in a microfluidic chip, which integrated a preformed graphitic carbon nitride photocatalyst (g-C3N4) and electron mediator (M) in one chip and mimicked the characteristics of photosystem I. The simultaneous assembly of g-C3N4and M could efficiently regenerate NADH from NAD+under visible light irradiation, which verified the effectiveness of the assembly method. The in situ assembly method was thought to outperform traditional methods in several aspects in terms of facile synthesis, promotion of the combination of g-C3N4and M through p-p stacking and an enhanced coenzyme regeneration rate. For comparison, we used the bulk g-C3N4-slurry and the few-layer g-C3N4-slurry system as the control to regenerate the photocatalytic cofactor/coenzyme NADH, and measured the required times of 305 s and 30 s, respectively, to accomplish 63% NAD+conversion. In contrast, our IAPSI microreactor takes only 13 s, faster than the other two by factors of 23 and 2.3 times. Therefore, we assert that the simple, yet highly efficient nature of this technique can act as an important method for artificial photosynthesis, particularly in the photocatalytic cofactor recycling systems for the production of various valuable molecules.
Showing items related by title, author, creator and subject.
Parand, R; Yao, Hong Mei; Pareek, Vishnu; Tade, Moses (2014)In this study, an Extended Composite Table Algorithm is developed to target the minimum freshwater, regenerated water, and wastewater flow rates together with the minimum regeneration concentration and wastewater concentration ...
Hou, Lei (2013)Assembly is the process in which two or more objects are joined together through particular sequences and operations. Current practice utilises two-dimensional (2D) drawings as the main visualisation means to guide assembly. ...
Di Marcantonio, D.; Galli, D.; Carubbi, C.; Gobbi, G.; Queirolo, V.; Martini, S.; Merighi, S.; Vaccarezza, Mauro; Maffulli, N.; Sykes, S.; Vitale, M.; Mirandola, P. (2015)Introduction: Satellite cells are muscle resident stem cells and are responsible for muscle regeneration. In this study we investigate the involvement of PKCε during muscle stem cell differentiation in vitro and in vivo. ...