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dc.contributor.authorSyme, Robert
dc.contributor.authorTan, Kar-Chun
dc.contributor.authorHane, James
dc.contributor.authorDodhia, Kejal
dc.contributor.authorStoll, T.
dc.contributor.authorHastie, M.
dc.contributor.authorFuruki, Eiko
dc.contributor.authorEllwood, Simon
dc.contributor.authorWilliams, A.
dc.contributor.authorTan, Y.
dc.contributor.authorRenwick, Alison
dc.contributor.authorGorman, J.
dc.contributor.authorOliver, Richard
dc.date.accessioned2017-01-30T10:57:42Z
dc.date.available2017-01-30T10:57:42Z
dc.date.created2016-09-22T12:29:03Z
dc.date.issued2016
dc.identifier.citationSyme, R. and Tan, K. and Hane, J. and Dodhia, K. and Stoll, T. and Hastie, M. and Furuki, E. et al. 2016. Comprehensive annotation of the Parastagonospora nodorum reference genome using next-generation genomics, transcriptomics and proteogenomics. PLoS One. 11 (2): e0147221.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/7114
dc.identifier.doi10.1371/journal.pone.0147221
dc.description.abstract

Parastagonospora nodorum, the causal agent of Septoria nodorum blotch (SNB), is an economically important pathogen of wheat (Triticum spp.), and a model for the study of necrotrophic pathology and genome evolution. The reference P. nodorum strain SN15 was the first Dothideomycete with a published genome sequence, and has been used as the basis for comparison within and between species. Here we present an updated reference genome assembly with corrections of SNP and indel errors in the underlying genome assembly from deep resequencing data as well as extensive manual annotation of gene models using transcriptomic and proteomic sources of evidence (https://github.com/robsyme/Parastagonospora_nodorum_SN15). The updated assembly and annotation includes 8,366 genes with modified protein sequence and 866 new genes. This study shows the benefits of using a wide variety of experimental methods allied to expert curation to generate a reliable set of gene models.

dc.publisherPublic Library of Science
dc.subjectFungal genomics
dc.subjectgene annotation
dc.subjectParastagonospora nodorum
dc.titleComprehensive annotation of the Parastagonospora nodorum reference genome using next-generation genomics, transcriptomics and proteogenomics
dc.typeJournal Article
dcterms.source.issn1932-6203
dcterms.source.titlePLoS One
curtin.note

This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

curtin.departmentDepartment of Environment and Agriculture
curtin.accessStatusOpen access


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