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    Fine-scale diet of the Australian sea lion (Neophoca cinerea) using DNA-based analysis of faeces

    Access Status
    Fulltext not available
    Authors
    Peters, K.
    Ophelkeller, K.
    Bott, N.
    Deagle, B.
    Jarman, Simon
    Goldsworthy, S.
    Date
    2015
    Type
    Journal Article
    
    Metadata
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    Citation
    Peters, K. and Ophelkeller, K. and Bott, N. and Deagle, B. and Jarman, S. and Goldsworthy, S. 2015. Fine-scale diet of the Australian sea lion (Neophoca cinerea) using DNA-based analysis of faeces. Marine Ecology. 36 (3): pp. 347-367.
    Source Title
    Marine Ecology
    DOI
    10.1111/maec.12145
    ISSN
    0173-9565
    School
    School of Molecular and Life Sciences (MLS)
    URI
    http://hdl.handle.net/20.500.11937/71298
    Collection
    • Curtin Research Publications
    Abstract

    © 2015 Blackwell Verlag GmbH. We applied DNA-based faecal analysis to determine the diet of female Australian sea lions (n = 12) from two breeding colonies in South Australia. DNA dietary components of fish and cephalopods were amplified using the polymerase chain reaction and mitochondrial DNA primers targeting the short (~100 base pair) section of the 16S gene region. Prey diversity was determined by sequencing ~50 amplicons generated from clone libraries developed for each individual. Faecal DNA was also combined and cloned from multiple individuals at each colony and fish diversity determined. Diets varied between individuals and sites. Overall, DNA analysis identified a broad diversity of prey comprising 23 fish and five cephalopod taxa, including many species not previously described as prey of the Australian sea lion. Labridae (wrasse), Monacanthidae (leatherjackets) and Mullidae (goat fish) were important fish prey taxa. Commonly identified cephalopods were Octopodidae (octopus), Loliginidae (calamary squid) and Sepiidae (cuttlefish). Comparisons of fish prey diversity determined by pooling faecal DNA from several samples provided a reasonable but incomplete resemblance (55-71%) to the total fish diversity identified across individual diets at each site. Interpretation of diet based on the recovery of prey hard-parts identified one cephalopod beak (Octopus sp.) and one fish otolith (Parapriacanthus elongatus). The present study highlights the value of DNA-based analyses and their capabilities to enhance information of trophic interactions.

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