Intracellular speciation of gold nanorods alters the conformational dynamics of genomic DNA
Embargo Lift Date
MetadataShow full item record
© 2018, The Author(s), under exclusive licence to Springer Nature Limited. Gold nanorods are one of the most widely explored inorganic materials in nanomedicine for diagnostics, therapeutics and sensing1. It has been shown that gold nanorods are not cytotoxic and localize within cytoplasmic vesicles following endocytosis, with no nuclear localization2,3, but other studies have reported alterations in gene expression profiles in cells following exposure to gold nanorods, via unknown mechanisms4. In this work we describe a pathway that can contribute to this phenomenon. By mapping the intracellular chemical speciation process of gold nanorods, we show that the commonly used Au–thiol conjugation, which is important for maintaining the noble (inert) properties of gold nanostructures, is altered following endocytosis, resulting in the formation of Au(i)–thiolates that localize in the nucleus5. Furthermore, we show that nuclear localization of the gold species perturbs the dynamic microenvironment within the nucleus and triggers alteration of gene expression in human cells. We demonstrate this using quantitative visualization of ubiquitous DNA G-quadruplex structures, which are sensitive to ionic imbalances, as an indicator of the formation of structural alterations in genomic DNA.
Showing items related by title, author, creator and subject.
Timing and Duration of High-Temperature Gold Mineralization and Spatially Associated Granitoid Magmatism at Chalice, Yilgarn Craton, Western AustraliaBucci, L.; McNaughton, Neal; Fletcher, Ian; Groves, D.; Kositcin, N.; Stein, H.; Hagemann, S. (2004)The Chalice gold deposit, in the Eastern Goldfields province, Yilgarn Craton, Western Australia, is located in a middle to upper amphibolite facies metamorphic domain and is hosted by a mafic-ultramafic rock sequence that ...
Chenrai, Piyaphong (2008)The Comet Gold Mine is in the Murchison mineral field which lies within the Yilgarn Craton of Western Australia. Several different geophysical methods were used in this study to define the geophysical signatures of ...
Structural and geochronological studies on the Liba goldfield of the West Qinling Orogen, Central ChinaZeng, Qing-Tao; McCuaig, T; Hart, Craig; Jourdan, Fred; Muhling, Janet; Bagas, Leon (2012)The Liba goldfield, located to the northeast of the Zhongchuan Granite in the West Qinling Orogen (WQO) of mainland China, contains the largest known gold resource of 2.8 Moz in the Zhongchuan area. Devonian metasedimentary ...