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    Intravital multiphoton imaging of the selective uptake of water-dispersible quantum dots into sinusoidal liver cells

    Access Status
    Fulltext not available
    Authors
    Liang, X.
    Grice, J.
    Zhu, Y.
    Liu, D.
    Sanchez, W.
    Li, Z.
    Crawford, D.
    Le Couteur, D.
    Cogger, V.
    Liu, Jian
    Xu, Z.
    Roberts, M.
    Date
    2015
    Type
    Journal Article
    
    Metadata
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    Citation
    Liang, X. and Grice, J. and Zhu, Y. and Liu, D. and Sanchez, W. and Li, Z. and Crawford, D. et al. 2015. Intravital multiphoton imaging of the selective uptake of water-dispersible quantum dots into sinusoidal liver cells. Small. 11 (14): pp. 1711-1720.
    Source Title
    Small
    DOI
    10.1002/smll.201402698
    ISSN
    1613-6810
    School
    WASM: Minerals, Energy and Chemical Engineering (WASM-MECE)
    URI
    http://hdl.handle.net/20.500.11937/71463
    Collection
    • Curtin Research Publications
    Abstract

    © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Although many studies reporting the organ-level biodistribution of nanoparticles (NPs) in animals, very few have addressed the fate of NPs in organs at the cellular level. The liver appears to be the main organ for accumulation of NPs after intravenous injection. In this study, for the first time, the in vivo spatiotemporal disposition of recently developed mercaptosuccinic acid (MSA)-capped cadmium telluride/cadmium sulfide (CdTe/CdS) quantum dots (QDs) is explored in rat liver using multiphoton microscopy (MPM) coupled with fluorescence lifetime imaging (FLIM), with subcellular resolution (1 µm). With high fluorescence efficiency and largely improved stability in the biological environment, these QDs show a distinct distribution pattern in the liver compared to organic dyes, rhodamine 123 and fluorescein. After intravenous injection, fluorescent molecules are taken up by hepatocytes and excreted into the bile, while negatively charged QDs are retained in the sinusoids and selectively taken up by sinusoidal cells (Kupffer cells and liver sinusoidal endothelial cells), but not by hepatocytes within 3 h. The results could help design NPs targeting the specific types of liver cells and choose the fluorescent markers for appropriate cellular imaging. Spatiotemporal disposition of water dispersible quantum dots (QDs) is explored in liver in vivo using multiphoton microscopy with fluorescence lifetime imaging. After intravenous injection, negatively charged QDs are distributed in the sinusoids and selectively taken up by sinusoidal cells, while fluorescent molecules (rhodamine 123 and fluorescein) are taken up by hepatocytes and excreted into the bile.

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