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    Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization

    Access Status
    Fulltext not available
    Authors
    Chao, C.
    Zhang, B.
    Zhai, R.
    Xiang, X.
    Liu, Jian
    Chen, R.
    Date
    2014
    Type
    Journal Article
    
    Metadata
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    Citation
    Chao, C. and Zhang, B. and Zhai, R. and Xiang, X. and Liu, J. and Chen, R. 2014. Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization. ACS Sustainable Chemistry and Engineering. 2 (3): pp. 396-403.
    Source Title
    ACS Sustainable Chemistry and Engineering
    DOI
    10.1021/sc400199v
    ISSN
    2168-0485
    School
    WASM: Minerals, Energy and Chemical Engineering (WASM-MECE)
    URI
    http://hdl.handle.net/20.500.11937/71716
    Collection
    • Curtin Research Publications
    Abstract

    Inorganic nanostructures and their assemblies play important roles in immobilizing biomolecules. Herein, we developed a facile and green methodology to assemble natural halloysite nanotubes (1D building blocks) into nest-like porous microspheres (3D architecture). We further modified the microspheres with dopamine to form a biomimetic entity. The interconnected and hierarchical pores within the microspheres provide larger pore volume to entrap biomolecules, and the abundant functional groups on the pore surface bond covalently with enzyme to enhance the immobilization ability. The porous microspheres showed excellent loading capacity for laccase immobilization as high as 311.2 mg/g, around 30 times higher than the individual halloysite nanotubes (11.3 mg/g). The specific activity above 80% was retained for the immobilized laccase compared to the free laccase. In addition, the immobilized enzyme exhibited remarkable thermal and recycle use stability. The biomimetic microspheres are expected to be biologically safe and chemically stable microcapsules for immobilizing a variety of biomolecules because of their natural and biofriendly characteristics. © 2013 American Chemical Society.

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