Heat-dried sclerotia of Sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect Brassica napus
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The phytopathogenic fungus Sclerotinia sclerotiorum forms dormant structures (termed sclerotia) that germinate myceliogenically under certain environmental conditions. During myceliogenic germination, sclerotia produce hyphae, which can infect leaves or stems of host plants directly from the ground; this is termed basal infection. This study determined which abiotic conditions were most important for promoting myceliogenic germination of sclerotia in vitro. A high sclerotium hydration level and low incubation temperature (158C) improved mycelial growth in the presence of a nutrient source. Sclerotia incubated without a nutrient source on moist sand, vigorously myceliogenically germinated most frequently (63%) when they had been previously imbibed and then conditioned at -20°C. By far the most consistent amount of vigorous myceliogenic germination (>75%) was produced when sclerotia were heat-dried before being submerged in water. The hyphae of these sclerotia were shown to infect and proliferate on leaves of intact Brassica napus plants. This research provides a better understanding of the abiotic conditions that are likely to increase the risk of basal infection by S. sclerotiorum.
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Bennett, Sarita ; Derbyshire, Mark; Michael, Pippa; Denton-Giles, Matthew; Lui, king yin; Thomson, linda (2019)Key messages • Preconditioning temperatures between 35 and 50°C for 30 to 60 days increases sclerotia germination to 80%, compared to with no preconditioning • Sclerotinia stem rot infections were low in field trials ...
Carpogenic germinability of diverse Sclerotinia sclerotorium (Lib.) de Bary populations within the south-western Australian grain beltMichael, Pippa ; Lui, King-Yin; Thomson, Linda; Stefanova, Katia; Bennett, Sarita (2020)Sclerotinia stem rot, caused by the necrotrophic plant pathogen Sclerotinia sclerotorium (Lib.) de Bary, is a major disease of canola and pulses in Australia. Current disease management relies greatly on cultural and ...
The control of sclerotinia stem rot on oilseed rape (Brassica napus): current practices and future opportunitiesDerbyshire, Mark; Denton-Giles, Matthew (2016)Sclerotinia stem rot (SSR) caused by the phytopathogenic fungus Sclerotinia sclerotiorum is a major disease of oilseed rape (Brassica napus). During infection, large, white/grey lesions form on the stems of the host plant, ...