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    Case study: Applying rapid flow cytometry analysis to CAPD effluent

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    Fulltext not available
    Authors
    Mulroney, K.
    Hall, J.
    McGuire, A.
    Inglis, T.
    Chakera, Aron
    Date
    2018
    Type
    Journal Article
    
    Metadata
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    Citation
    Mulroney, K. and Hall, J. and McGuire, A. and Inglis, T. and Chakera, A. 2018. Case study: Applying rapid flow cytometry analysis to CAPD effluent. Peritoneal Dialysis International. 38 (5): pp. 376-379.
    Source Title
    Peritoneal Dialysis International
    DOI
    10.3747/pdi.2017.00231
    ISSN
    0896-8608
    School
    Curtin Medical School
    URI
    http://hdl.handle.net/20.500.11937/72333
    Collection
    • Curtin Research Publications
    Abstract

    © 2018, Multimed Inc.. All rights reserved. Peritoneal dialysis (PD) peritonitis cases require rapid clinical interventions to ensure the best possible patient outcomes. Culture-dependent microbiology tools are slow and cannot provide clinicians with evidence to guide antimicrobial prescription practices in an appropriate time frame. Genotypic methods have met with limited success for analyzing continuous ambulatory PD effluent, with most centers still relying on culture-dependent microbiology. We present a case study in which we apply flow cytometry techniques to antibiotic-compromised effluent. We demonstrate, with supporting evidence, direct enumeration of bacterial and human immune cells, with results reported within 2 hours of receiving the clinical specimen.

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