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    Surveillance for azole resistance in clinical and environmental isolates of Aspergillus fumigatus in Australia and cyp51A homology modelling of azole-resistant isolates

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    Authors
    Talbot, J.
    Subedi, S.
    Halliday, C.
    Hibbs, D.
    Lai, F.
    Lopez-Ruiz, Fran
    Harper, L.
    Park, R.
    Cuddy, W.
    Biswas, C.
    Cooley, L.
    Carter, D.
    Sorrell, T.
    Barrs, V.
    Chen, S.
    Date
    2018
    Type
    Journal Article
    
    Metadata
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    Citation
    Talbot, J. and Subedi, S. and Halliday, C. and Hibbs, D. and Lai, F. and Lopez-Ruiz, F. and Harper, L. et al. 2018. Surveillance for azole resistance in clinical and environmental isolates of Aspergillus fumigatus in Australia and cyp51A homology modelling of azole-resistant isolates. The Journal of antimicrobial chemotherapy. 73 (9): pp. 2347-2351.
    Source Title
    The Journal of antimicrobial chemotherapy
    DOI
    10.1093/jac/dky187
    ISSN
    0305-7453
    School
    Centre for Crop and Disease Management (CCDM)
    URI
    http://hdl.handle.net/20.500.11937/73283
    Collection
    • Curtin Research Publications
    Abstract

    Background: The prevalence of azole resistance in Aspergillus fumigatus is uncertain in Australia. Azole exposure may select for resistance. We investigated the frequency of azole resistance in a large number of clinical and environmental isolates. Methods: A. fumigatus isolates [148 human, 21 animal and 185 environmental strains from air (n=6) and azole-exposed (n=64) or azole-naive (n=115) environments] were screened for azole resistance using the VIPcheckTM system. MICs were determined using the SensititreTM YeastOne YO10 assay. Sequencing of the Aspergillus cyp51A gene and promoter region was performed for azole-resistant isolates, and cyp51A homology protein modelling undertaken. Results: Non-WT MICs/MICs at the epidemiological cut-off value of one or more azoles were observed for 3/148 (2%) human isolates but not amongst animal, or environmental, isolates. All three isolates grew on at least one azole-supplemented well based on VIPcheckTM screening. For isolates 9 and 32, the itraconazole and posaconazole MICs were 1 mg/L (voriconazole MICs 0.12 mg/L); isolate 129 had itraconazole, posaconazole and voriconazole MICs of >16, 1 and 8 mg/L, respectively. Soil isolates from azole-exposed and azole-naive environments had similar geometric mean MICs of itraconazole, posaconazole and voriconazole (P>0.05). A G54R mutation was identified in the isolates exhibiting itraconazole and posaconazole resistance, and the TR34/L98H mutation in the pan-azole-resistant isolate. cyp51A modelling predicted that the G54R mutation would prevent binding of itraconazole and posaconazole to the haem complex. Conclusions: Azole resistance is uncommon in Australian clinical and environmental A. fumigatus isolates; further surveillance is indicated.

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