DNA metabarcoding and the cytochrome c oxidase subunit I marker: Not a perfect match
MetadataShow full item record
© 2014 The Author(s) Published by the Royal Society. DNA metabarcoding enables efficient characterization of species composition in environmental DNA or bulk biodiversity samples, and this approach is making significant and unique contributions in the field of ecology. In metabarcoding of animals, the cytochrome c oxidase subunit I (COI) gene is frequently used as the marker of choice because no other genetic region can be found in taxonomically verified databases with sequences covering so many taxa. However, the accuracy of metabarcoding datasets is dependent on recovery of the targeted taxa using conserved amplification primers.We argue that COI does not contain suitably conserved regions for most amplicon-based metabarcoding applications. Marker selection deserves increased scrutiny and available marker choices should be broadened in order to maximize potential in this exciting field of research.
Showing items related by title, author, creator and subject.
Parducci, L.; Matetovici, I.; Fontana, S.; Bennett, K.; Suyama, Y.; Haile, James; Kjær, K.; Larsen, N.; Drouzas, A.; Willerslev, E. (2013)Plant and animal biodiversity can be studied by obtaining DNA directly from the environment. This new approach in combination with the use of generic barcoding primers (metabarcoding) has been suggested as complementary ...
Environmental DNA (eDNA) metabarcoding reveals strong discrimination among diverse marine habitats connected by water movementJeunen, G.; Knapp, M.; Spencer, H.; Lamare, M.; Taylor, H.; Stat, M.; Bunce, Michael; Gemmell, N. (2018)While in recent years environmental DNA (eDNA) metabarcoding surveys have shown great promise as an alternative monitoring method, the integration into existing marine monitoring programs may be confounded by the dispersal ...
Advancing DNA barcoding and metabarcoding applications for plants requires systematic analysis of herbarium collections-an Australian perspectiveDormontt, E.; van Dijk, K.; Bell, K.; Biffin, E.; Breed, M.; Byrne, M.; Caddy-Retalic, S.; Encinas-Viso, F.; Nevill, Paul; Shapcott, A.; Young, J.; Waycott, M.; Lowe, A. (2018)Building DNA barcode databases for plants has historically been ad hoc, and often with a relatively narrow taxonomic focus. To realize the full potential of DNA barcoding for plants, and particularly its application to ...