Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System
Access Status
Open access
Authors
Arooj, Mahira
Date
2018Supervisor
Ricardo Mancera
Type
Thesis
Award
PhD
Metadata
Show full item recordFaculty
Science and Engineering
School
Pharmacy and Biomedical Sciences
Collection
Abstract
This research aimed to repress target genes by modulating their expression through rewriting of aberrant epigenetic landscapes, including DNA methylation and histone post-translational modifications, utilizing epigenetic repressor domains. The epigenetic repressor domains included KRAB, DNMT3A, UHRF1 and CSD, which were delivered via the epigenetic editing tools ZFs, TALEs and the CRISPR/dCas9 system. Our findings suggest that a combination of multiple epigenetic repressor domains targeting epigenetic marks involved in epigenetic cross-talk significantly repressed target genes.
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