Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System
| dc.contributor.author | Arooj, Mahira | |
| dc.contributor.supervisor | Ricardo Mancera | en_US |
| dc.date.accessioned | 2019-01-29T07:45:43Z | |
| dc.date.available | 2019-01-29T07:45:43Z | |
| dc.date.issued | 2018 | en_US |
| dc.identifier.uri | http://hdl.handle.net/20.500.11937/73571 | |
| dc.description.abstract |
This research aimed to repress target genes by modulating their expression through rewriting of aberrant epigenetic landscapes, including DNA methylation and histone post-translational modifications, utilizing epigenetic repressor domains. The epigenetic repressor domains included KRAB, DNMT3A, UHRF1 and CSD, which were delivered via the epigenetic editing tools ZFs, TALEs and the CRISPR/dCas9 system. Our findings suggest that a combination of multiple epigenetic repressor domains targeting epigenetic marks involved in epigenetic cross-talk significantly repressed target genes. | en_US |
| dc.publisher | Curtin University | en_US |
| dc.title | Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System | en_US |
| dc.type | Thesis | en_US |
| dcterms.educationLevel | PhD | en_US |
| curtin.department | Pharmacy and Biomedical Sciences | en_US |
| curtin.accessStatus | Open access | en_US |
| curtin.faculty | Science and Engineering | en_US |