Development and validation of a simple LC-MS/MS method for the simultaneous quantitative determination of trimethylamine-N-oxide and branched chain amino acids in human serum
dc.contributor.author | Le, T. | |
dc.contributor.author | Shafaei, A. | |
dc.contributor.author | Genoni, A. | |
dc.contributor.author | Christophersen, Claus | |
dc.contributor.author | Devine, A. | |
dc.contributor.author | Lo, J. | |
dc.contributor.author | Wall, P. | |
dc.contributor.author | Boyce, M. | |
dc.date.accessioned | 2019-02-19T04:15:15Z | |
dc.date.available | 2019-02-19T04:15:15Z | |
dc.date.created | 2019-02-19T03:58:32Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Le, T. and Shafaei, A. and Genoni, A. and Christophersen, C. and Devine, A. and Lo, J. and Wall, P. et al. 2019. Development and validation of a simple LC-MS/MS method for the simultaneous quantitative determination of trimethylamine-N-oxide and branched chain amino acids in human serum. Analytical and Bioanalytical Chemistry. 411 (5): pp. 1019-1028. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/73913 | |
dc.identifier.doi | 10.1007/s00216-018-1522-8 | |
dc.description.abstract |
Serum branched chain amino acids and trimethylamine-N-oxide are monitored as potential indicators of diabetes and cardiovascular health respectively. A rapid method for their simultaneous determination using liquid chromatography and tandem mass spectrometry is described here. Branched chain amino acids and trimethylamine-N-oxide were quantified based on their specific MS/MS fragments using a selected reaction monitoring approach. A number of columns were tested for their ability to separate the analytes. A C18-PFP column separated the analytes in just 4 minutes, and resulted in excellent peak shape and retention time repeatability, and was therefore chosen as the optimal column. A second column, the Intrada Amino Acid column, was chosen for comparison and validation experiments as it provided an orthogonal separation mechanism. The intra-day and inter-day precision and accuracy were less than 12% for trimethylamine-N-oxide and less than 6% for the branched chain amino acids. Recoveries, where serum was spiked with three different concentrations of the analytes, ranged from 97 to 113%. The LODs and LOQs for trimethylamine-N-oxide were 1 and 6 ng/mL, for leucine and isoleucine were 4 and 8 ng/mL, and for valine were 5 and 15 ng/mL, respectively. The C18-PFP column method was validated using the Intrada Amino Acid column method and percentage agreement for all four analytes was within 10%. Sample preparation was minimal, and use of labelled internal standards accounted for matrix effects. The method was successfully applied to human plasma samples. | |
dc.publisher | Springer | |
dc.title | Development and validation of a simple LC-MS/MS method for the simultaneous quantitative determination of trimethylamine-N-oxide and branched chain amino acids in human serum | |
dc.type | Journal Article | |
dcterms.source.volume | 411 | |
dcterms.source.number | 5 | |
dcterms.source.startPage | 1019 | |
dcterms.source.endPage | 1028 | |
dcterms.source.issn | 1618-2642 | |
dcterms.source.title | Analytical and Bioanalytical Chemistry | |
curtin.department | School of Molecular and Life Sciences (MLS) | |
curtin.accessStatus | Fulltext not available |
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