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    Challenges for assessing vertebrate diversity in turbid Saharan water-bodies using environmental DNA

    Access Status
    Fulltext not available
    Authors
    Egeter, B.
    Peixoto, S.
    Brito, J.
    Jarman, Simon
    Puppo, P.
    Velo-Antón, G.
    Date
    2018
    Type
    Journal Article
    
    Metadata
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    Citation
    Egeter, B. and Peixoto, S. and Brito, J. and Jarman, S. and Puppo, P. and Velo-Antón, G. 2018. Challenges for assessing vertebrate diversity in turbid Saharan water-bodies using environmental DNA. Genome. 61 (11): pp. 807-814.
    Source Title
    Genome
    DOI
    10.1139/gen-2018-0071
    ISSN
    0831-2796
    School
    School of Molecular and Life Sciences (MLS)
    URI
    http://hdl.handle.net/20.500.11937/74743
    Collection
    • Curtin Research Publications
    Abstract

    The Sahara desert is the largest warm desert in the world and a poorly explored area. Small water-bodies occur across the desert and are crucial habitats for vertebrate biodiversity. Environmental DNA (eDNA) is a powerful tool for species detection and is being increasingly used to conduct biodiversity assessments. However, there are a number of difficulties with sampling eDNA from such turbid water-bodies and it is often not feasible to rely on electrical tools in remote desert environments. We trialled a manually powered filtering method in Mauritania, using pre-filtration to circumvent problems posed by turbid water in remote arid areas. From nine vertebrate species expected in the water-bodies, four were detected visually, two via metabarcoding, and one via both methods. Difficulties filtering turbid water led to severe constraints, limiting the sampling protocol to only one sampling point per study site, which alone may largely explain why many of the expected vertebrate species were not detected. The amplification of human DNA using general vertebrate primers is also likely to have contributed to the low number of taxa identified. Here we highlight a number of challenges that need to be overcome to successfully conduct metabarcoding eDNA studies for vertebrates in desert environments in Africa.

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