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dc.contributor.authorFonceca, A.M.
dc.contributor.authorZosky, G.R.
dc.contributor.authorBozanich, E.M.
dc.contributor.authorSutanto, E.N.
dc.contributor.authorKicic, Anthony
dc.contributor.authorMcNamara, P.S.
dc.contributor.authorKnight, D.A.
dc.contributor.authorSly, P.D.
dc.contributor.authorTurner, D.J.
dc.contributor.authorStick, S.M.
dc.date.accessioned2019-11-09T20:32:28Z
dc.date.available2019-11-09T20:32:28Z
dc.date.issued2018
dc.identifier.citationFonceca, A.M. and Zosky, G.R. and Bozanich, E.M. and Sutanto, E.N. and Kicic, A. and McNamara, P.S. and Knight, D.A. et al. 2018. Accumulation mode particles and LPS exposure induce TLR-4 dependent and independent inflammatory responses in the lung. Respiratory Research. 19 (1): ARTN 15.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/76775
dc.identifier.doi10.1186/s12931-017-0701-z
dc.description.abstract

© 2018 The Author(s). Background: Accumulation mode particles (AMP) are formed from engine combustion and make up the inhalable vapour cloud of ambient particulate matter pollution. Their small size facilitates dispersal and subsequent exposure far from their original source, as well as the ability to penetrate alveolar spaces and capillary walls of the lung when inhaled. A significant immuno-stimulatory component of AMP is lipopolysaccharide (LPS), a product of Gram negative bacteria breakdown. As LPS is implicated in the onset and exacerbation of asthma, the presence or absence of LPS in ambient particulate matter (PM) may explain the onset of asthmatic exacerbations to PM exposure. This study aimed to delineate the effects of LPS and AMP on airway inflammation, and potential contribution to airways disease by measuring airway inflammatory responses induced via activation of the LPS cellular receptor, Toll-like receptor 4 (TLR-4). Methods: The effects of nebulized AMP, LPS and AMP administered with LPS on lung function, cellular inflammatory infiltrate and cytokine responses were compared between wildtype mice and mice not expressing TLR-4. Results: The presence of LPS administered with AMP appeared to drive elevated airway resistance and sensitivity via TLR-4. Augmented TLR4 driven eosinophilia and greater TNF-α responses observed in AMP-LPS treated mice independent of TLR-4 expression, suggests activation of allergic responses by TLR4 and non-TLR4 pathways larger than those induced by LPS administered alone. Treatment with AMP induced macrophage recruitment independent of TLR-4 expression. Conclusions: These findings suggest AMP-LPS as a stronger stimulus for allergic inflammation in the airways then LPS alone.

dc.languageEnglish
dc.publisherBIOMED CENTRAL LTD
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectRespiratory System
dc.subjectAsthma
dc.subjectTLR-4
dc.subjectPM
dc.subjectLPS
dc.subjectAMP
dc.subjectCOPD
dc.subjectAMBIENT PARTICULATE MATTER
dc.subjectAIR-POLLUTION
dc.subjectASTHMA
dc.subjectINHALATION
dc.subjectMICE
dc.subjectTOXICITY
dc.subjectURBAN
dc.subjectLIPOPOLYSACCHARIDE
dc.subjectEXPRESSION
dc.subjectULTRAFINE
dc.titleAccumulation mode particles and LPS exposure induce TLR-4 dependent and independent inflammatory responses in the lung
dc.typeJournal Article
dcterms.source.volume19
dcterms.source.number1
dcterms.source.issn1465-9921
dcterms.source.titleRespiratory Research
dc.date.updated2019-11-09T20:32:24Z
curtin.departmentSchool of Public Health
curtin.accessStatusOpen access
curtin.facultyFaculty of Health Sciences
curtin.contributor.orcidKicic, Anthony [0000-0002-0008-9733]
curtin.identifier.article-numberARTN 15
dcterms.source.eissn1465-993X
curtin.contributor.scopusauthoridKicic, Anthony [6507472922]


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