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dc.contributor.authorBanerjee, B.
dc.contributor.authorKicic, Anthony
dc.contributor.authorMusk, M.
dc.contributor.authorSutanto, E.N.
dc.contributor.authorStick, S.M.
dc.contributor.authorChambers, D.C.
dc.date.accessioned2019-11-10T02:25:31Z
dc.date.available2019-11-10T02:25:31Z
dc.date.issued2009
dc.identifier.citationBanerjee, B. and Kicic, A. and Musk, M. and Sutanto, E.N. and Stick, S.M. and Chambers, D.C. 2009. Successful establishment of primary small airway cell cultures in human lung transplantation. Respiratory Research. 10: ARTN 99.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/76820
dc.identifier.doi10.1186/1465-9921-10-99
dc.description.abstract

Background: The study of small airway diseases such as post-transplant bronchiolitis obliterans syndrome (BOS) is hampered by the difficulty in assessing peripheral airway function either physiologically or directly. Our aims were to develop robust methods for sampling small airway epithelial cells (SAEC) and to establish submerged SAEC cultures for downstream experimentation.Methods: SAEC were obtained at 62 post-transplant bronchoscopies in 26 patients using radiologically guided bronchial brushings. Submerged cell cultures were established and SAEC lineage was confirmed using expression of clara cell secretory protein (CCSP).Results: The cell yield for SAEC (0.956 ± 0.063 × 106) was lower than for large airway cells (1.306 ± 0.077 × 106) but did not significantly impact on the culture establishment rate (79.0 ± 5.2% vs. 83.8 ± 4.7% p = 0.49). The presence of BOS significantly compromised culture success (independent of cell yield) for SAEC (odds ratio (95%CI) 0.067 (0.01-0.40)) but not LAEC (0.3 (0.05-1.9)). Established cultures were successfully passaged and expanded.Conclusion: Primary SAEC can be successfully obtained from human lung transplant recipients and maintained in culture for downstream experimentation. This technique will facilitate the development of primary in vitro models for BOS and other diseases with a small airway component such as asthma, cystic fibrosis and COPD. © 2009 Banerjee et al; licensee BioMed Central Ltd.

dc.languageEnglish
dc.publisherBMC
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectRespiratory System
dc.subjectBRONCHIOLITIS-OBLITERANS-SYNDROME
dc.subjectBRONCHIAL EPITHELIAL-CELLS
dc.subjectOBSTRUCTIVE PULMONARY-DISEASE
dc.subjectMESENCHYMAL TRANSITION
dc.subjectINCREASED EXPRESSION
dc.subjectTOBACCO SMOKERS
dc.subjectRECIPIENTS
dc.titleSuccessful establishment of primary small airway cell cultures in human lung transplantation
dc.typeJournal Article
dcterms.source.volume10
dcterms.source.issn1465-9921
dcterms.source.titleRespiratory Research
dc.date.updated2019-11-10T02:25:26Z
curtin.departmentSchool of Public Health
curtin.accessStatusOpen access
curtin.facultyFaculty of Health Sciences
curtin.contributor.orcidKicic, Anthony [0000-0002-0008-9733]
curtin.identifier.article-numberARTN 99
dcterms.source.eissn1465-993X
curtin.contributor.scopusauthoridKicic, Anthony [6507472922]


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