Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Trehalose biosynthesis is involved in sporulation of Stagonospora nodorum

    171236_171236.pdf (635.2Kb)
    Access Status
    Open access
    Authors
    Lowe, R.
    Lord, M.
    Rybak, K.
    Trengove, R.
    Oliver, Richard
    Solomon, P.
    Date
    2009
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Lowe, Rohan G.T. and Lord, Maryn and Rybak, Kasia and Trengove, Robert D. and Oliver, Richard P. and Solomon, Peter S. 2009. Trehalose biosynthesis is involved in sporulation of Stagonospora nodorum. Fungal Genetics and Biology. 46 (5): pp. 381-389.
    Source Title
    Fungal Genetics and Biology
    DOI
    10.1016/j.fgb.2009.02.002
    ISSN
    1087-1845
    School
    Department of Environment and Agriculture
    Remarks

    NOTICE: this is the author’s version of a work that was accepted for publication in Fungal Genetics and Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Genetics and Biology, Vol. 46 (5) May 2009. http://dx.doi.org/10.1016/j.fgb.2009.02.002

    URI
    http://hdl.handle.net/20.500.11937/7925
    Collection
    • Curtin Research Publications
    Abstract

    Stagonospora nodorum is a necrotrophic fungal pathogen that is the causal agent of leaf and glume blotch on wheat. S. nodorum is a polycyclic pathogen, whereby rain-splashed pycnidiospores attach to and colonise wheat tissue and subsequently sporulate again within 2–3 weeks. As several cycles of infection are needed for a damaging infection, asexual sporulation is a critical phase of its infection cycle. A non-targeted metabolomics screen for sporulation-associated metabolites identified that trehalose accumulated significantly in concert with asexual sporulation both in vitro and in planta. A reverse-genetics approach was used to investigate the role of trehalose in asexual sporulation. Trehalose biosynthesis was disrupted by deletion of the gene Tps1, encoding a trehalose 6-phosphate synthase, resulting in almost total loss of trehalose during in vitro growth and in planta. In addition, lesion development and pycnidia formation were also significantly reduced in tps1 mutants. Reintroduction of the Tps1 gene restored trehalose biosynthesis, pathogenicity and sporulation to wild-type levels. Microscopic examination of tps1 infected wheat leaves showed that pycnidial formation often halted at an early stage of development.Further examination of the tps1 phenotype revealed that tps1 pycnidiospores exhibited a reduced germination rate while under heat stress, and tps1 mutants had a reduced growth rate while under oxidative stress. This study confirms a link between trehalose biosynthesis and pathogen fitness in S.nodorum.

    Related items

    Showing items related by title, author, creator and subject.

    • Investigating the role of calcium/calmodulin-dependent protein kinases in Stagonospora nodorum
      Solomon, P.; Rybak, K.; Trengove, R.; Oliver, Richard (2006)
      Three genes encoding different Ca2+/calmodulin-dependent protein kinases have been characterized in the wheat phytopathogenic fungus Stagonospora nodorum. The kinases were identified from the S. nodorum genome sequence ...
    • Investigating the role of calcium/calmodulin-dependent protein kinases in Stagonospora nodorum
      Solomon, P.; Rybak, K.; Trengove, R.; Oliver, Richard (2006)
      Three genes encoding different Ca2+/calmodulin-dependent protein kinases have been characterized in the wheat phytopathogenic fungus Stagonospora nodorum. The kinases were identified from the S. nodorum genome sequence ...
    • Functional characterisation of glyoxalase I from the fungal wheat pathogen Stagonospora nodorum
      Solomon, P.; Oliver, Richard (2004)
      During an expressed sequence tag sequencing project, a gene encoding a methylglyoxal lyase (glyoxalase I) was identified, cloned and characterised from the necrotrophic wheat pathogen Stagonospora nodorum. Sequence analysis ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.