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dc.contributor.authorNyagwange, J.
dc.contributor.authorTijhaar, E.
dc.contributor.authorTernette, N.
dc.contributor.authorMobegi, Fredrick
dc.contributor.authorTretina, K.
dc.contributor.authorSilva, J.C.
dc.contributor.authorPelle, R.
dc.contributor.authorNene, V.
dc.date.accessioned2020-08-24T06:52:25Z
dc.date.available2020-08-24T06:52:25Z
dc.date.issued2018
dc.identifier.citationNyagwange, J. and Tijhaar, E. and Ternette, N. and Mobegi, F. and Tretina, K. and Silva, J.C. and Pelle, R. et al. 2018. Characterization of the Theileria parva sporozoite proteome. International Journal for Parasitology. 48 (3-4): pp. 265-273.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/80731
dc.identifier.doi10.1016/j.ijpara.2017.09.007
dc.description.abstract

East Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovine lymphocytes. Blocking this initial stage of invasion presents a promising vaccine strategy for control of East Coast fever and can in part be achieved by targeting the major sporozoite surface protein p67. To support research on the biology of T. parva and the identification of additional candidate vaccine antigens, we report on the sporozoite proteome as defined by LC–MS/MS analysis. In total, 4780 proteins were identified in an enriched preparation of sporozoites. Of these, 2007 were identified as T. parva proteins, representing close to 50% of the total predicted parasite proteome. The remaining 2773 proteins were derived from the tick vector. The identified sporozoite proteins include a set of known T. parva antigens targeted by antibodies and cytotoxic T cells from cattle that are immune to East Coast fever. We also identified proteins predicted to be orthologs of Plasmodium falciparum sporozoite surface molecules and invasion organelle proteins, and proteins that may contribute to the phenomenon of bovine lymphocyte transformation. Overall, these data establish a protein expression profile of T. parva sporozoites as an important starting point for further study of a parasitic species which has considerable agricultural impact.

dc.languageEnglish
dc.publisherELSEVIER SCI LTD
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectParasitology
dc.subjectTheileria
dc.subjectSporozoites
dc.subjectProteomics
dc.subjectMudPIT
dc.subjectAntigens
dc.subjectEast Coast fever
dc.subjectEAST-COAST FEVER
dc.subjectPOLYMORPHIC IMMUNODOMINANT MOLECULE
dc.subjectCANDIDATE VACCINE ANTIGENS
dc.subjectAPICAL MEMBRANE ANTIGEN-1
dc.subjectT-CELL-CLONES
dc.subjectPLASMODIUM-FALCIPARUM
dc.subjectMALARIA PARASITE
dc.subjectSURFACE-ANTIGEN
dc.subjectINFECTED LYMPHOCYTES
dc.subjectMONOCLONAL-ANTIBODY
dc.titleCharacterization of the Theileria parva sporozoite proteome
dc.typeJournal Article
dcterms.source.volume48
dcterms.source.number3-4
dcterms.source.startPage265
dcterms.source.endPage273
dcterms.source.issn0020-7519
dcterms.source.titleInternational Journal for Parasitology
dc.date.updated2020-08-24T06:52:22Z
curtin.note

© 2018 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology.

curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidMobegi, Fredrick [0000-0003-0554-9919]
curtin.contributor.researcheridMobegi, Fredrick [D-1058-2015]
dcterms.source.eissn1879-0135
curtin.contributor.scopusauthoridMobegi, Fredrick [56479121000]


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