Show simple item record

dc.contributor.authorTaylor, S.L.
dc.contributor.authorLeong, L.E.X.
dc.contributor.authorMobegi, Fredrick
dc.contributor.authorChoo, J.M.
dc.contributor.authorBurr, L.D.
dc.contributor.authorWesselingh, S.
dc.contributor.authorRogers, G.B.
dc.identifier.citationTaylor, S.L. and Leong, L.E.X. and Mobegi, F.M. and Choo, J.M. and Burr, L.D. and Wesselingh, S. and Rogers, G.B. 2018. Understanding the impact of antibiotic therapies on the respiratory tract resistome: A novel pooled-template metagenomic sequencing strategy. Multidisciplinary Respiratory Medicine. 13 (Suppl 1): Article No. 30.

Determining the effects of antimicrobial therapies on airway microbiology at a population-level is essential. Such analysis allows, for example, surveillance of antibiotic-induced changes in pathogen prevalence, the emergence and spread of antibiotic resistance, and the transmission of multi-resistant organisms. However, current analytical strategies for understanding these processes are limited. Culture- and PCR-based assays for specific microbes require the a priori selection of targets, while antibiotic sensitivity testing typically provides no insight into either the molecular basis of resistance, or the carriage of resistance determinants by the wider commensal microbiota. Shotgun metagenomic sequencing provides an alternative approach that allows the microbial composition of clinical samples to be described in detail, including the prevalence of resistance genes and virulence traits. While highly informative, the application of metagenomics to large patient cohorts can be prohibitively expensive. Using sputum samples from a randomised placebo-controlled trial of erythromycin in adults with bronchiectasis, we describe a novel, cost-effective strategy for screening patient cohorts for changes in resistance gene prevalence. By combining metagenomic screening of pooled DNA extracts with validatory quantitative PCR-based analysis of candidate markers in individual samples, we identify population-level changes in the relative abundance of specific macrolide resistance genes. This approach has the potential to provide an important adjunct to current analytical strategies, particularly within the context of antimicrobial clinical trials.

dc.subjectAntibiotic therapy
dc.subjectMetagenomic screening
dc.titleUnderstanding the impact of antibiotic therapies on the respiratory tract resistome: A novel pooled-template metagenomic sequencing strategy
dc.typeJournal Article
dcterms.source.numberSuppl 1
dcterms.source.titleMultidisciplinary Respiratory Medicine

© The Author(s). 2018 Published in Multidisciplinary Respiratory Medicine.

curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidMobegi, Fredrick [0000-0003-0554-9919]
curtin.contributor.researcheridMobegi, Fredrick [D-1058-2015]
curtin.contributor.scopusauthoridMobegi, Fredrick [56479121000]

Files in this item


This item appears in the following Collection(s)

Show simple item record
Except where otherwise noted, this item's license is described as