Show simple item record

dc.contributor.authorKarunarathne, Sakura D
dc.contributor.authorHan, Yong
dc.contributor.authorZhang, Xiao-Qi
dc.contributor.authorZhou, Gaofeng
dc.contributor.authorHill, Camilla B
dc.contributor.authorChen, Kefei
dc.contributor.authorAngessa, Tefera
dc.contributor.authorLi, Chengdao
dc.identifier.citationKarunarathne, S.D. and Han, Y. and Zhang, X.-Q. and Zhou, G. and Hill, C.B. and Chen, K. and Angessa, T. et al. 2020. Genome-Wide Association Study and Identification of Candidate Genes for Nitrogen Use Efficiency in Barley (Hordeum vulgare L.) Frontiers in Plant Science. 11: Article No. 571912.

Nitrogen (N) fertilizer is largely responsible for barley grain yield potential and quality, yet excessive application leads to environmental pollution and high production costs. Therefore, efficient use of N is fundamental for sustainable agriculture. In the present study, we investigated the performance of 282 barley accessions through hydroponic screening using optimal and low NH<sub>4</sub>NO<sub>3</sub> treatments. Low-N treatment led to an average shoot dry weight reduction of 50%, but there were significant genotypic differences among the accessions. Approximately 20% of the genotypes showed high (>75%) relative shoot dry weight under low-N treatment and were classified as low-N tolerant, whereas 20% were low-N sensitive (≤55%). Low-N tolerant accessions exhibited well-developed root systems with an average increase of 60% in relative root dry weight to facilitate more N absorption. A genome-wide association study (GWAS) identified 66 significant marker trait associations (MTAs) conferring high nitrogen use efficiency, four of which were stable across experiments. These four MTAs were located on chromosomes 1H(1), 3H(1), and 7H(2) and were associated with relative shoot length, relative shoot and root dry weight. Genes corresponding to the significant MTAs were retrieved as candidate genes, including members of the asparagine synthetase gene family, several transcription factor families, protein kinases, and nitrate transporters. Most importantly, the high-affinity nitrate transporter 2.7 (HvNRT2.7) was identified as a promising candidate on 7H for root and shoot dry weight. The identified candidate genes provide new insights into our understanding of the molecular mechanisms driving nitrogen use efficiency in barley and represent potential targets for genetic improvement.

dc.titleGenome-Wide Association Study and Identification of Candidate Genes for Nitrogen Use Efficiency in Barley (Hordeum vulgare L.)
dc.typeJournal Article
dcterms.source.titleFrontiers in Plant Science

© 2020 The Authors. Published in Frontiers in Plant Science

curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidChen, Kefei [0000-0002-2380-3805]

Files in this item


This item appears in the following Collection(s)

Show simple item record
Except where otherwise noted, this item's license is described as