Show simple item record

dc.contributor.authorChan, L.
dc.contributor.authorCross, H.F.
dc.contributor.authorShe, J.K.
dc.contributor.authorCavalli, G.
dc.contributor.authorMartins, H.F.P.
dc.contributor.authorNeylon, Cameron
dc.contributor.editorKoutsopoulos, Sotirios
dc.date.accessioned2020-10-21T10:27:11Z
dc.date.available2020-10-21T10:27:11Z
dc.date.issued2007
dc.identifier.citationChan, L. and Cross, H.F. and She, J.K. and Cavalli, G. and Martins, H.F.P. and Neylon, C. 2007. Covalent attachment of proteins to solid supports and surfaces via sortase-mediated ligation. PLoS ONE. 2 (11): Article No. e1164.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/81471
dc.identifier.doi10.1371/journal.pone.0001164
dc.description.abstract

Background. There is growing interest in the attachment of proteins to solid supports for the development of supported catalysts, affinity matrices, and micro devices as well as for the development of planar and bead based protein arrays for multiplexed assays of protein concentration, interactions, and activity. A critical requirement for these applications is the generation of a stable linkage between the solid support and the immobilized, but still functional, protein.

Methodology. Solid supports including crosslinked polymer beads, beaded agarose, and planar glass surfaces, were modified to present an oligoglycine motif to solution. A range of proteins were ligated to the various surfaces using the Sortase A enzyme of S. aureus. Reactions were carried out in aqueous buffer conditions at room temperature for times between one and twelve hours.

Conclusions. The Sortase A transpeptidase of S. aureus provides a general, robust, and gentle approach to the selective covalent immobilization of proteins on three very different solid supports. The proteins remain functional and accessible to solution. Sortase mediated ligation is therefore a straightforward methodology for the preparation of solid supported enzymes and bead based assays, as well as the modification of planar surfaces for microanalytical devices and protein arrays.

dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectScience & Technology
dc.subjectMultidisciplinary Sciences
dc.subjectScience & Technology - Other Topics
dc.subjectSTAPHYLOCOCCUS-AUREUS
dc.subjectESCHERICHIA-COLI
dc.subjectIMMOBILIZATION
dc.subjectMUTANT
dc.subjectCHIP
dc.subjectTUS
dc.titleCovalent attachment of proteins to solid supports and surfaces via sortase-mediated ligation
dc.typeJournal Article
dcterms.source.volume2
dcterms.source.number11
dcterms.source.issn1932-6203
dcterms.source.titlePLoS ONE
dc.date.updated2020-10-21T10:27:10Z
curtin.note

© 2007 Chan et al.

curtin.departmentSchool of Media, Creative Arts and Social Inquiry
curtin.accessStatusOpen access
curtin.facultyFaculty of Humanities
curtin.contributor.orcidNeylon, Cameron [0000-0002-0068-716X]
curtin.contributor.researcheridNeylon, Cameron [B-6265-2008]
curtin.identifier.article-numberARTN e1164
dcterms.source.eissn1932-6203
curtin.contributor.scopusauthoridNeylon, Cameron [9738760800]


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

http://creativecommons.org/licenses/by/4.0/
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/