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dc.contributor.authorWhiteford, N.
dc.contributor.authorHaslam, N.
dc.contributor.authorWeber, G.
dc.contributor.authorPrügel-Bennett, A.
dc.contributor.authorEssex, J.W.
dc.contributor.authorRoach, P.L.
dc.contributor.authorBradley, M.
dc.contributor.authorNeylon, Cameron
dc.date.accessioned2020-10-21T10:28:27Z
dc.date.available2020-10-21T10:28:27Z
dc.date.issued2005
dc.identifier.citationWhiteford, N. and Haslam, N. and Weber, G. and Prügel-Bennett, A. and Essex, J.W. and Roach, P.L. and Bradley, M. et al. 2005. An analysis of the feasibility of short read sequencing. Nucleic Acids Research. 33 (19): Article No. e171.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/81475
dc.identifier.doi10.1093/nar/gni170
dc.description.abstract

Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20-30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1.

dc.languageeng
dc.publisherOxford University Press
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectChromosomes, Human, Pair 1
dc.subjectFeasibility Studies
dc.subjectGenome, Bacterial
dc.subjectGenome, Human
dc.subjectGenome, Viral
dc.subjectGenomics
dc.subjectHumans
dc.subjectSequence Analysis, DNA
dc.titleAn analysis of the feasibility of short read sequencing.
dc.typeJournal Article
dcterms.source.volume33
dcterms.source.number19
dcterms.source.startPagee171
dcterms.source.issn1362-4962
dcterms.source.titleNucleic Acids Research
dc.date.updated2020-10-21T10:28:27Z
curtin.note

© The Author 2005. Published in Nucleic Acids Research by Oxford University Press.

curtin.departmentSchool of Media, Creative Arts and Social Inquiry
curtin.accessStatusOpen access
curtin.facultyFaculty of Humanities
curtin.contributor.orcidNeylon, Cameron [0000-0002-0068-716X]
curtin.contributor.researcheridNeylon, Cameron [B-6265-2008]
dcterms.source.eissn1362-4962
curtin.contributor.scopusauthoridNeylon, Cameron [9738760800]


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