Investigating Changes to the Post Translational Modification Status of Key Components of the ER Translocation Machinery in Saccharomyces Cerevisiae

Abstract

Dysfunction of the endoplasmic reticulum is commonly observed in disease, however, the exact nature in which this manifests requires further investigation. We have examined the role post translational modifications have in regulating multiple aspects of cellular biology using the model organism, Saccharomyces cerevisiae. This work highlights the complex nature of the endoplasmic reticulum, elucidating novel mechanisms whereby phosphorylation and glycosylation are able to modulate activity in response to changes in the cellular environment.