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dc.contributor.authorJacques, Silke
dc.contributor.authorLenzo, Leon
dc.contributor.authorStevens, Kofi
dc.contributor.authorLawrence, Julie
dc.contributor.authorTan, Kar-Chun
dc.date.accessioned2021-05-21T14:23:55Z
dc.date.available2021-05-21T14:23:55Z
dc.date.issued2021
dc.identifier.citationJacques, S. and Lenzo, L. and Stevens, K. and Lawrence, J. and Tan, K.-C. 2021. An optimized sporulation method for the wheat fungal pathogen Pyrenophora tritici-repentis. Plant Methods. 17. Article No. 52.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/83687
dc.identifier.doi10.1186/s13007-021-00751-4
dc.description.abstract

Background:

The necrotrophic fungal pathogen Pyrenophora tritici-repentis (Ptr) causes tan (syn. yellow) spot of wheat and accounts for significant yield losses worldwide. Understanding the molecular mechanisms of this economically important crop disease is crucial to counteract the yield and quality losses of wheat globally. Substantial progress has been made to comprehend the race structure of this phytopathogen based on its production of necrotrophic effectors and genomic resources of Ptr. However, one limitation for studying Ptr in a laboratory environment is the difficulty to isolate high spore numbers from vegetative growth with mycelial contamination common. These limitations reduce the experimental tractability of Ptr.

Results:

Here, we optimized a multitude of parameters and report a sporulation method for Ptr that yields robust, high quality and pure spores. Our methodology encompasses simple and reproducible plugging and harvesting techniques, resulting in spore yields up to 1500 fold more than the current sporulation methods and was tested on multiple isolates and races of Ptr as well as an additional seven modern Australian Ptr isolates. Moreover, this method also increased purity and spore harvest numbers for two closely related fungal pathogens (Pyrenophora teres f. maculata and f. teres) that cause net blotch diseases in barley (Hordeum vulgare), highlighting the usability of this optimized sporulation protocol for the wider research community.

Conclusions:

Large-scale spore infection and virulence assays are essential for the screening of wheat and barley cultivars and combined with the genetic mapping of these populations allows pinpointing and exploiting sources of host genetic resistance. We anticipate that improvements in spore numbers and purity will further advance research to increase our understanding of the pathogenicity mechanisms of these important fungal pathogens.

dc.publisherBioMed Central
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleAn optimized sporulation method for the wheat fungal pathogen Pyrenophora tritici-repentis
dc.typeJournal Article
dcterms.source.issn1746-4811
dcterms.source.titlePlant Methods
dc.date.updated2021-05-21T14:23:54Z
curtin.note

© The Author(s). 2021 Published in Plant Methods. This article is published under the Open Access publishing model and distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/. Please refer to the licence to obtain terms for any further reuse or distribution of this work.

curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidTan, Kar-Chun [0000-0001-6094-823X]
curtin.contributor.orcidStevens, Kofi [0000-0002-3373-5471]
curtin.contributor.orcidLenzo, Leon [0000-0002-4071-4708]
curtin.contributor.scopusauthoridTan, Kar-Chun [56231324800] [7403999337]


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