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dc.contributor.authorMoolhuijzen, Paula
dc.contributor.authorSee, Pao Theen
dc.contributor.authorMoffat, Caroline
dc.date.accessioned2022-02-04T07:38:51Z
dc.date.available2022-02-04T07:38:51Z
dc.date.issued2019
dc.identifier.citationMoolhuijzen, P. and See, P.T. and Moffat, C.S. 2019. A new PacBio genome sequence of an Australian Pyrenophora tritici-repentis race 1 isolate. BMC Research Notes. 12 (1): Article No. 642.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/87570
dc.identifier.doi10.1186/s13104-019-4681-6
dc.description.abstract

Objectives: The necrotrophic fungal pathogen Pyrenophora tritici-repentis (Ptr) is the causal agent of tan spot a major disease of wheat. We have generated a new genome resource for an Australian Ptr race 1 isolate V1 to support comparative 'omics analyses. In particular, the V1 PacBio Biosciences long-read sequence assembly was generated to confirm the stability of large-scale genome rearrangements of the Australian race 1 isolate M4 when compared to the North American race 1 isolate Pt-1C-BFP.

Results: Over 1.3 million reads were sequenced by PacBio Sequel small-molecule real-time sequencing (SRMT) cell to yield 11.4 Gb for the genome assembly of V1 (285X coverage), with median and maximum read lengths of 8959 bp and 72,292 bp respectively. The V1 genome was assembled into 33 contiguous sequences with a of total length 40.4 Mb and GC content of 50.44%. A total of 14,050 protein coding genes were predicted and annotated for V1. Of these 11,519 genes were orthologous to both Pt-1C-BFP and M4. Whole genome alignment of the Australian long-read assemblies (V1 to M4) confirmed previously identified large-scale genome rearrangements between M4 and Pt-1C-BFP and presented small scale variations, which included a sequence break within a race-specific region for ToxA, a well-known necrotrophic effector gene.

dc.languageeng
dc.publisherBioMed Central
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectComparative analysis
dc.subjectGenome assembly
dc.subjectPacBio sequel
dc.subjectPyrenophora tritici-repentis
dc.subjectTan spot
dc.subjectToxA
dc.subjectYellow spot
dc.subjectAscomycota
dc.subjectAustralia
dc.subjectChromosome Mapping
dc.subjectFungal Proteins
dc.subjectGene Expression Regulation, Fungal
dc.subjectGenome, Fungal
dc.subjectHigh-Throughput Nucleotide Sequencing
dc.subjectHost-Pathogen Interactions
dc.subjectMycotoxins
dc.subjectOpen Reading Frames
dc.subjectPlant Diseases
dc.subjectTriticum
dc.titleA new PacBio genome sequence of an Australian Pyrenophora tritici-repentis race 1 isolate
dc.typeJournal Article
dcterms.source.volume12
dcterms.source.number1
dcterms.source.startPage642
dcterms.source.issn1756-0500
dcterms.source.titleBMC Research Notes
dc.date.updated2022-02-04T07:38:50Z
curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidMoolhuijzen, Paula [0000-0002-3502-7612]
curtin.contributor.orcidMoffat, Caroline [0000-0002-6360-1933]
curtin.contributor.researcheridMoffat, Caroline [I-1058-2014]
dcterms.source.eissn1756-0500
curtin.contributor.scopusauthoridMoolhuijzen, Paula [14525407700]
curtin.contributor.scopusauthoridSee, Pao Theen [56231274400]
curtin.contributor.scopusauthoridMoffat, Caroline [25936541300]


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