Correction to: PacBio genome sequencing reveals new insights into the genomic organisation of the multi-copy ToxB gene of the wheat fungal pathogen Pyrenophora tritici-repentis

Abstract

Following publication of the original article [1], it was reported that an update on the location of DW5 ToxB cluster relative to the M4 chromosome 10 fusion event was required. Consequently, the Abstract, Table 2, Fig. 7 and several relevant sections of the article have been updated. The changes are given in this Correction article with the updated text highlighted in bold face. Abstract A total of ten identical ToxB gene copies were identified and based on flanking sequence identity, nine loci appeared associated with chromosome 11 and a single copy with chromosome 5. Chromosome 11 multiple ToxB gene loci were separated by large sequence regions between 31 - 66 kb within larger segmental duplications in an alternating pattern related to loci strand, and flanked by transposable elements. Whole genome comparative analysis between Ptr races 1 and 5 A chromosome fusion between chromosome 10 and 11 (referred to as chromosome 10) in Australian isolate M4 resolved by optical mapping [2] was not observed for DW5, where DW5 contig 8 possessed both 5’ and 3’ telomere motifs (Table 2), which would represent a chromosome (telomere to telomere). Ptr ToxB multiloci analysis In this study, the ToxB loci were located on chromosome 5 and 11, which had assembly sizes of 3.36 and 2.18 Mb respectively, which are close to the previously estimated chromosome sizes by Martinez et. al, (2004). Of the ten ToxB loci, nine appeared to be associated with the smaller chromosome 11 located in the 3’ distal region. A Ptr chromosome noted for a chromosome fusion event for a race 1 isolate M4 [2]. The telomere to telomere support for eleven DW5 chromosomes is similar to the findings for another American race 1 isolate Ptr Pt-1C-BFP [3], unlike the 10 chromosome genome of Australian isolate M4 (1) (Fig. 7). (Table presented.). (Figure presented.). Ptr ToxB patterns of duplication In addition to the positioning of the ToxB duplication within the distal region of chromosome 11, ToxB loci were located equidistant downstream from dimer TnphaT transposases, a familiar gene found coupled to Ptr ToxA and within the horizontally transferred region, also found in Parastagonospora nodorum and Bipolaris sorokiniana [4, 5].