Fabrication of selective l-glutamic acid sensor in electrochemical technique from wet-chemically prepared RuO2 doped ZnO nanoparticles
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In this approach, RuO2 doped ZnO nanoparticles (NPs; 10% RuO2 doping) were prepared by wet-chemical method and characterized by powder X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Energy-dispersive X-ray spectroscopy (EDS), and Field Emission Scanning Electron Microscopy (FESEM). The slurry of RuO2-doped ZnO NPs in ethanol was deposited as thin film of onto a glassy carbon electrode (GCE) to result in a working electrode to be used for L-glutamic acid (L-GA) sensing probe. The electrochemical response of the sensor was found to be linear in the range of 0.1 nM–0.01 mM in current versus logarithm of concentration plot, called ‘linear dynamic range (LDR)’. The sensitivity of the electrode is found to be 5.42 μAμM−1cm−2. The detection limit is estimated to be 96.0 ± 5.0 pM by using signal-noise ratio of 3. The proposed L-GA sensor has shown excellent reproducibility, good stability and fast response time. It is successfully used to analyze the selective biological sample with the RuO2-doped ZnO nanoparticles fabricated sensor matrix. Thus, this methodology for developing enzyme-less sensor would be the most reliable, efficient, and simple route in the field of healthcare sector in broad scales.
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