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dc.contributor.authorLamichhane, Hum Bahadur
dc.contributor.authorHenares, Terence G
dc.contributor.authorHackett, Mark
dc.contributor.authorArrigan, Damien
dc.date.accessioned2022-11-03T13:08:28Z
dc.date.available2022-11-03T13:08:28Z
dc.date.issued2021
dc.identifier.citationLamichhane, H.B. and Henares, T.G. and Hackett, M.J. and Arrigan, D.W.M. 2021. Structural Changes in Insulin at a Soft Electrochemical Interface. Analytical Chemistry. 93 (26): pp. 9094-9102.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/89593
dc.identifier.doi10.1021/acs.analchem.1c00657
dc.description.abstract

Understanding the interaction of proteins at interfaces, which occurs at or within cell membranes and lipoprotein vesicles, is central to our understanding of protein function. Therefore, new experimental approaches to understand how protein structure is influenced by protein-interface interactions are important. Herein we build on our previous work exploring electrochemistry at the interface between two immiscible electrolyte solutions (ITIES) to investigate changes in protein secondary structure that are modulated by protein-interface interactions. The ITIES provides an experimental framework to drive protein adsorption at an interface, allowing subsequent spectroscopic analysis (e.g., Fourier transform infrared spectroscopy) to monitor changes in protein structure. Here, we reveal that the interaction between insulin and the interface destabilizes native insulin secondary structure, promoting formation of α helix secondary structures. These structural alterations result from protein-interface rather than protein-protein interactions at the interface. Although this is an emerging approach, our results provide a foundation highlighting the value of the ITIES as a tool to study protein structure and interactions at interfaces. Such knowledge may be useful to elucidate protein function within biological systems or to aid sensor development.

dc.languageEnglish
dc.publisherAMER CHEMICAL SOC
dc.subjectScience & Technology
dc.subjectPhysical Sciences
dc.subjectChemistry, Analytical
dc.subjectChemistry
dc.subject2 IMMISCIBLE ELECTROLYTES
dc.subjectEGG-WHITE-LYSOZYME
dc.subjectSECONDARY STRUCTURE
dc.subjectADSORPTION-KINETICS
dc.subjectCIRCULAR-DICHROISM
dc.subjectAMYLOID FIBRILS
dc.subjectPROTEINS
dc.subjectBEHAVIOR
dc.subjectFTIR
dc.subjectSPECTROSCOPY
dc.titleStructural Changes in Insulin at a Soft Electrochemical Interface
dc.typeJournal Article
dcterms.source.volume93
dcterms.source.number26
dcterms.source.startPage9094
dcterms.source.endPage9102
dcterms.source.issn0003-2700
dcterms.source.titleAnalytical Chemistry
dc.date.updated2022-11-03T13:08:27Z
curtin.note

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.1c00657

curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidHackett, Mark [0000-0002-3296-7270]
curtin.contributor.orcidArrigan, Damien [0000-0002-1053-1273]
curtin.contributor.researcheridArrigan, Damien [A-7440-2010]
dcterms.source.eissn1520-6882
curtin.contributor.scopusauthoridHackett, Mark [35240056500]
curtin.contributor.scopusauthoridArrigan, Damien [7004238830]


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