“Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy
dc.contributor.author | Khambatta, Karina | |
dc.contributor.author | Hollings, Ashley | |
dc.contributor.author | Sauzier, Georgina | |
dc.contributor.author | Sanglard, Lilian M.V.P. | |
dc.contributor.author | Klein, A.R. | |
dc.contributor.author | Tobin, M.J. | |
dc.contributor.author | Vongsvivut, J. | |
dc.contributor.author | Gibberd, Mark | |
dc.contributor.author | Payne, Alan | |
dc.contributor.author | Naim, Fatima | |
dc.contributor.author | Hackett, Mark | |
dc.date.accessioned | 2023-01-24T04:32:14Z | |
dc.date.available | 2023-01-24T04:32:14Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Khambatta, K. and Hollings, A. and Sauzier, G. and Sanglard, L.M.V.P. and Klein, A.R. and Tobin, M.J. and Vongsvivut, J. et al. 2021. “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy. Advanced Science. 8 (19): ARTN 2101902. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/90105 | |
dc.identifier.doi | 10.1002/advs.202101902 | |
dc.description.abstract |
Analysis of the epicuticular wax layer on the surface of plant leaves can provide a unique window into plant physiology and responses to environmental stimuli. Well-established analytical methodologies can quantify epicuticular wax composition, yet few methods are capable of imaging wax distribution in situ or in vivo. Here, the first report of Fourier transform infrared (FTIR) reflectance spectroscopic imaging as a non-destructive, in situ, method to investigate variation in epicuticular wax distribution at 25 µm spatial resolution is presented. The authors demonstrate in vivo imaging of alterations in epicuticular waxes during leaf development and in situ imaging during plant disease or exposure to environmental stressors. It is envisaged that this new analytical capability will enable in vivo studies of plants to provide insights into how the physiology of plants and crops respond to environmental stresses such as disease, soil contamination, drought, soil acidity, and climate change. | |
dc.language | English | |
dc.publisher | WILEY | |
dc.relation.sponsoredby | http://purl.org/au-research/grants/arc/FT190100017 | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Science & Technology | |
dc.subject | Physical Sciences | |
dc.subject | Technology | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | Chemistry | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | Materials Science | |
dc.subject | epicuticular wax | |
dc.subject | infrared | |
dc.subject | microscopy | |
dc.subject | plant physiology | |
dc.subject | reflectance | |
dc.subject | SPECTROSCOPY | |
dc.subject | RAMAN | |
dc.subject | STRESS | |
dc.subject | ATR | |
dc.subject | INTERFACE | |
dc.subject | SURFACES | |
dc.title | “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy | |
dc.type | Journal Article | |
dcterms.source.volume | 8 | |
dcterms.source.number | 19 | |
dcterms.source.issn | 2198-3844 | |
dcterms.source.title | Advanced Science | |
dc.date.updated | 2023-01-24T04:32:14Z | |
curtin.department | School of Molecular and Life Sciences (MLS) | |
curtin.accessStatus | Open access | |
curtin.faculty | Faculty of Science and Engineering | |
curtin.contributor.orcid | Hollings, Ashley [0000-0001-7829-4932] | |
curtin.contributor.orcid | Khambatta, Karina [0000-0002-5012-4250] | |
curtin.contributor.orcid | Sauzier, Georgina [0000-0002-9908-7956] | |
curtin.contributor.orcid | Naim, Fatima [0000-0001-8451-1104] | |
curtin.contributor.orcid | Hackett, Mark [0000-0002-3296-7270] | |
curtin.contributor.orcid | Payne, Alan [0000-0003-4530-6348] | |
curtin.contributor.researcherid | Payne, Alan [G-4995-2015] | |
curtin.identifier.article-number | ARTN 2101902 | |
dcterms.source.eissn | 2198-3844 | |
curtin.contributor.scopusauthorid | Sauzier, Georgina [55600124800] | |
curtin.contributor.scopusauthorid | Hackett, Mark [35240056500] | |
curtin.contributor.scopusauthorid | Gibberd, Mark [6701329783] | |
curtin.contributor.scopusauthorid | Payne, Alan [8731082200] |