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    Construction of single-chain Fv with two possible CDR3H conformations but similar inter-molecular forces that neutralize bovine herpesvirus 1

    Access Status
    Fulltext not available
    Authors
    Koti, M.
    Farrugia, W.
    Nagy, E.
    Ramsland, Paul
    Kaushik, A.
    Date
    2010
    Type
    Journal Article
    
    Metadata
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    Citation
    Koti, M. and Farrugia, W. and Nagy, E. and Ramsland, P. and Kaushik, A. 2010. Construction of single-chain Fv with two possible CDR3H conformations but similar inter-molecular forces that neutralize bovine herpesvirus 1. Molecular Immunology. 47 (5): pp. 953-960.
    Source Title
    Molecular Immunology
    DOI
    10.1016/j.molimm.2009.11.011
    ISSN
    0161-5890
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/9046
    Collection
    • Curtin Research Publications
    Abstract

    Bovine herpesvirus 1 (BoHV-1) causes respiratory and genital diseases in cattle for which available vaccines do not confer adequate protection. Since passive immunization with antibodies permits disease prevention, single-chain fragment variable (scFv), originating from a monoclonal bovine IgG1 antibody against BoHV-1, were constructed and expressed in Pichia pastoris in V?-VH orientation via a flexible seven-amino acid linker. Similar to the intact IgG, the purified recombinant scFv neutralized BoHV-1 in vitro and recognized viral antigens in BoHV-1 infected MDBK cells by immunofluorescence. Homology modeling of the Fv predicts two distinct conformations for CDR3H. Firstly, a long protruding CDR3H conformation where no disulfide linkage occurred between two "non-canonical" Cys residues resulted in a large binding cavity between V? and VH. Secondly, a smaller potential antigen-binding cavity is predicted with a disulfide linkage between the two Cys residues of CDR3H creating a six-membered loop in the ascending polypeptide, which fitted into the space between V? and VH. Despite such potential configurational diversity of the antigen-binding site, the electrostatic surface potentials that would interact with the BoHV-1 epitope are largely similar for both the topographies where salt-bridge type electrostatic interactions likely occur at the edges of the binding site. Given that IgG1 antibody against BoHV-1 is clonally selected, it is likely that disulfide-stabilized broader and flatter surface topography is specifically generated to accommodate the predicted carbohydrate neutralizing B-epitope on BoHV-1. The specificity and neutralizing capacity for BoHV-1 of the scFv should make this bovine antibody fragment a useful diagnostic and potential therapeutic candidate for an important viral pathogen in cattle. © 2009 Elsevier Ltd. All rights reserved.

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