Show simple item record

dc.contributor.authorDugal, L.
dc.contributor.authorThomas, L.
dc.contributor.authorWilkinson, S.P.
dc.contributor.authorRichards, Zoe
dc.contributor.authorAlexander, J.B.
dc.contributor.authorAdam, Arne A.S.
dc.contributor.authorKennington, W.J.
dc.contributor.authorJarman, Simon
dc.contributor.authorRyan, N.M.
dc.contributor.authorBunce, Michael
dc.contributor.authorGilmour, J.P.
dc.identifier.citationDugal, L. and Thomas, L. and Wilkinson, S.P. and Richards, Z.T. and Alexander, J.B. and Adam, A.A.S. and Kennington, W.J. et al. 2022. Coral monitoring in northwest Australia with environmental DNA metabarcoding using a curated reference database for optimized detection. Environmental DNA. 4 (1): pp. 63-76.

The need for efficient and more accurate ways of monitoring threatened ecosystems is becoming increasingly urgent as climate change intensifies. Coral reefs are an example of an ecosystem in crisis, with widespread declines in coral cover and diversity documented over recent decades. Novel molecular approaches such as biomonitoring using environmental DNA (eDNA) from seawater samples show great potential to complement future coral reef monitoring programs, especially when used in combination with conventional methods. However, eDNA metabarcoding studies often rely on public databases (e.g., GenBank) for assigning taxonomy, which generally limits the number of sequences that can be taxonomically identified. The extent to which building reference tissue sequences improves taxonomic resolution has yet to be fully examined. Here, we combined traditional coral reef monitoring data with eDNA assessments derived from seawater collected at the highly diverse Rowley Shoals in Western Australia. Using two ITS2 assays developed to target basal metazoan DNA and a reference database spiked with 70 local coral specimens, we identified 37 genera and 40 species from 56 1 L seawater samples. We identified considerable overlap of taxa with visual survey data and showed that assignment of amplicon sequence variants was significantly improved when “spiking” the taxonomic classifier with curated sequences of locally collected species. Our findings showcase the potential of eDNA metabarcoding for monitoring the biodiversity of reef corals and highlight the importance of custom reference sequence databases for improving taxonomic resolution in metabarcoding studies.

dc.titleCoral monitoring in northwest Australia with environmental DNA metabarcoding using a curated reference database for optimized detection
dc.typeJournal Article
dcterms.source.titleEnvironmental DNA
curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidBunce, Michael [0000-0002-0302-4206]
curtin.contributor.orcidRichards, Zoe [0000-0002-8947-8996]
curtin.contributor.orcidJarman, Simon [0000-0002-0792-9686]
curtin.contributor.orcidAdam, Arne A.S. [0000-0002-2960-7880]
curtin.contributor.scopusauthoridBunce, Michael [55160482300]
curtin.contributor.scopusauthoridRichards, Zoe [23988153400]
curtin.contributor.scopusauthoridJarman, Simon [6701411935]

Files in this item


This item appears in the following Collection(s)

Show simple item record
Except where otherwise noted, this item's license is described as