Characterisation and optimisation of HIV-1-based SARS-CoV-2 
pseudovirus

Narayan, Maya

Narayan M 2025 Public.pdf (2.136Mb)

Access Status

Open access

Authors

Narayan, Maya

Date

2025

Supervisor

Carl Mousley

Padraig Strappe

Brian Brestovac

Type

Thesis

Award

MRes

Metadata

Show full item record

Faculty

Health Sciences

School

Curtin Medical School

URI

http://hdl.handle.net/20.500.11937/98199

Collection

Curtin Theses

Abstract

This study successfully generated high-titre HIV-1-based SARS-CoV-2 pseudovirus and characterised key factors influencing its infectivity including SARS-CoV-2 N incorporation. Optimising pseudovirus production and cell models can enhance assay efficiency and reproducibility, establishing a robust platform for SARS-CoV-2 research. These findings are valuable for the study of infectious diseases, serving not only as a model for spike-mediated entry but also as a tool for investigating the function of other structural proteins involved in viral assembly.