Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Quantitative proteomic analysis of G-protein signalling in Stagonospora nodorum using isobaric tags for relative and absolute quantification

    Access Status
    Fulltext not available
    Authors
    Casey, T.
    Solomon, P.
    Bringans, S.
    Tan, Kar-Chun
    Oliver, Richard
    Lipscombe, R.
    Date
    2010
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    CASEY T, SOLOMON PS, BRINGANS S, TAN K-C, OLIVER RP & LIPSCOMBE R (2010) Quantitative proteomic analysis of G-protein signalling in Stagonospora nodorum using isobaric tags for relative and absolute quantification. Proteomics. 10 38-47
    DOI
    10.1002/pmic.200900474
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/11305
    Collection
    • Curtin Research Publications
    Abstract

    The G protein α-subunit (Gna1) in the wheat pathogen Stagonospora nodorum has previously been shown to be a critical controlling element in disease ontogeny. In this study, iTRAQ and 2-D LC MALDI-MS/MS have been used to characterise protein expression changes in the S. nodorum gna1 strain versus the SN15 wild-type. A total of 1336 proteins were identified. The abundance of 49 proteins was significantly altered in the gna1 strain compared with the wild-type. Gna1 was identified as having a significant regulatory role on primary metabolic pathways, particularly those concerned with NADPH synthesis or consumption. Mannitol dehydrogenase was up-regulated in the gna1 strain while mannitol 1-phosphate dehydrogenase was down-regulated providing direct evidence of Gna1 regulation over this enigmatic pathway. Enzymatic analysis and growth assays confirmed this regulatory role. Several novel hypothetical proteins previously associated with stress and pathogen responses were identified as positively regulated by Gna1. A short-chain dehydrogenase (Sch3) was also significantly less abundant in the gna1 strains. Sch3 was further characterised by gene disruption in S. nodorum by homologous recombination. Functional characterisation of the sch3 strains revealed their inability to sporulate in planta providing a further link to Gna1 signalling and asexual reproduction. These data add significantly to the identification of the regulatory targets of Gna1 signalling in S. nodorum and have demonstrated the utility of iTRAQ in dissecting signal transduction pathways.

    Related items

    Showing items related by title, author, creator and subject.

    • Quantitative proteomic analysis of G-protein signallingin Stagonospora nodorum using isobaric tags forrelative and absolute quantification
      Casey, T.; Solomon, P.; Bringans, S.; Tan, Kar-Chun; Oliver, Richard; Lipscombe, R. (2010)
      The G protein a-subunit (Gna1) in the wheat pathogen Stagonospora nodorum has previouslybeen shown to be a critical controlling element in disease ontogeny. In this study, iTRAQ and2-D LC MALDI-MS/MS have been used to ...
    • A signalling-regulated short-chain dehydrogenase of Stagonospora nodorum regulates asexual development
      Tan, Kar-Chun; Heazlewood, J.; Millar, A.; Thomson, G.; Oliver, Richard; Solomon, P. (2008)
      The fungus Stagonospora nodorum is a causal agent of leaf and glume blotch disease of wheat. It has been previously shown that inactivation of heterotrimeric G protein signaling in Stagonospora nodorum caused development ...
    • A Signaling-Regulated, Short-Chain Dehydrogenase of Stagonospora nodorum Regulates Asexual Development
      Tan, K.; Heazlewood, J.; Millar, A.; Thomson, G.; Oliver, Richard; Solomon, P. (2008)
      The fungus Stagonospora nodorum is a causal agent of leaf and glume blotch disease of wheat. It has been previously shown that inactivation of heterotrimeric G protein signaling in Stagonospora nodorum caused development ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.