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    Quantitative proteomic analysis of G-protein signallingin Stagonospora nodorum using isobaric tags forrelative and absolute quantification

    Access Status
    Fulltext not available
    Authors
    Casey, T.
    Solomon, P.
    Bringans, S.
    Tan, Kar-Chun
    Oliver, Richard
    Lipscombe, R.
    Date
    2010
    Type
    Journal Article
    
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    Citation
    Casey, T. and Solomon, P. and Bringans, S. and Tan, K. and Oliver, R. and Lipscombe, R. 2010. Quantitative proteomic analysis of G-protein signallingin Stagonospora nodorum using isobaric tags forrelative and absolute quantification. Proteomics. 10: pp. 38-47.
    Source Title
    Proteomics
    ISSN
    16159853
    URI
    http://hdl.handle.net/20.500.11937/23148
    Collection
    • Curtin Research Publications
    Abstract

    The G protein a-subunit (Gna1) in the wheat pathogen Stagonospora nodorum has previouslybeen shown to be a critical controlling element in disease ontogeny. In this study, iTRAQ and2-D LC MALDI-MS/MS have been used to characterise protein expression changes in the S.nodorum gna1 strain versus the SN15 wild-type. A total of 1336 proteins were identified. Theabundance of 49 proteins was significantly altered in the gna1 strain compared with the wildtype.Gna1 was identified as having a significant regulatory role on primary metabolicpathways, particularly those concerned with NADPH synthesis or consumption. Mannitoldehydrogenase was up-regulated in the gna1 strain while mannitol 1-phosphate dehydrogenasewas down-regulated providing direct evidence of Gna1 regulation over this enigmaticpathway. Enzymatic analysis and growth assays confirmed this regulatory role. Severalnovel hypothetical proteins previously associated with stress and pathogen responses wereidentified as positively regulated by Gna1. A short-chain dehydrogenase (Sch3) was alsosignificantly less abundant in the gna1 strains. Sch3 was further characterised by genedisruption in S. nodorum by homologous recombination. Functional characterisation of thesch3 strains revealed their inability to sporulate in planta providing a further link to Gna1signalling and asexual reproduction. These data add significantly to the identification of theregulatory targets of Gna1 signalling in S. nodorum and have demonstrated the utility ofiTRAQ in dissecting signal transduction pathways.

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    • Quantitative proteomic analysis of G-protein signalling in Stagonospora nodorum using isobaric tags for relative and absolute quantification
      Casey, T.; Solomon, P.; Bringans, S.; Tan, Kar-Chun; Oliver, Richard; Lipscombe, R. (2010)
      The G protein α-subunit (Gna1) in the wheat pathogen Stagonospora nodorum has previously been shown to be a critical controlling element in disease ontogeny. In this study, iTRAQ and 2-D LC MALDI-MS/MS have been used to ...
    • A signalling-regulated short-chain dehydrogenase of Stagonospora nodorum regulates asexual development
      Tan, Kar-Chun; Heazlewood, J.; Millar, A.; Thomson, G.; Oliver, Richard; Solomon, P. (2008)
      The fungus Stagonospora nodorum is a causal agent of leaf and glume blotch disease of wheat. It has been previously shown that inactivation of heterotrimeric G protein signaling in Stagonospora nodorum caused development ...
    • A Signaling-Regulated, Short-Chain Dehydrogenase of Stagonospora nodorum Regulates Asexual Development
      Tan, K.; Heazlewood, J.; Millar, A.; Thomson, G.; Oliver, Richard; Solomon, P. (2008)
      The fungus Stagonospora nodorum is a causal agent of leaf and glume blotch disease of wheat. It has been previously shown that inactivation of heterotrimeric G protein signaling in Stagonospora nodorum caused development ...
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