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dc.contributor.authorFunnekotter, B.
dc.contributor.authorWhiteley, S.
dc.contributor.authorTurner, S.
dc.contributor.authorBunn, E.
dc.contributor.authorMancera, Ricardo
dc.date.accessioned2017-01-30T11:24:36Z
dc.date.available2017-01-30T11:24:36Z
dc.date.created2015-10-29T04:08:41Z
dc.date.issued2015
dc.identifier.citationFunnekotter, B. and Whiteley, S. and Turner, S. and Bunn, E. and Mancera, R. 2015. Evaluation of the new vacuum infiltration vitrification (viv) cryopreservation technique for native Australian plant shoot tips. Cryo letters. 36 (2): pp. 104-113.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/11419
dc.description.abstract

BACKGROUND: The application of a vacuum during the incubation in cryoprotective agents such as PVS2 allows for increased penetration, reducing total incubation times required before vitrification and post-cryopreservation regeneration is achieved. OBJECTIVE: This study compared a conventional droplet-vitrification protocol to the new vacuum infiltration vitrification protocol in four Australian plant species. MATERIALS AND METHODS: The new vacuum infiltration vitrification applied an 80 kPa vacuum during incubations in loading solution and PVS2. Infiltration of the cryoprotective agents into shoot tips was determined by differential scanning calorimetry measuring ice formation in the thermographs comparing a range of loading solution and PVS2 incubation times. RESULTS AND CONCLUSION: The application of the vacuum infiltration vitrification technique resulted in a significantly reduced PVS2 incubation time for cryogenic survival and regeneration for all four species, reducing the time needed to adequately protect shoot tips by half to a quarter when compared to a conventional droplet-vitrification technique.

dc.titleEvaluation of the new vacuum infiltration vitrification (viv) cryopreservation technique for native Australian plant shoot tips
dc.typeJournal Article
dcterms.source.volume36
dcterms.source.number2
dcterms.source.startPage104
dcterms.source.endPage113
dcterms.source.issn0143-2044
dcterms.source.titleCryo letters
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusFulltext not available


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