Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Cryopreservation of lomandra sonderi (asparagaceae) shoot tips using droplet-vitrification

    Access Status
    Fulltext not available
    Authors
    Menon, Akshay
    Funnekotter, Bryn
    Kaczmarczyk, Anja
    Bunn, E.
    Turner, S.
    Mancera, Ricardo
    Date
    2012
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Menon, A. and Funnekotter, B. and Kaczmarczyk, A. and Bunn, E. and Turner, S. and Mancera, R. 2012. Cryopreservation of lomandra sonderi (asparagaceae) shoot tips using droplet-vitrification. Cryo-Letters. 33 (4): pp. 259-270.
    Source Title
    Cryo-Letters
    ISSN
    01432044
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/7486
    Collection
    • Curtin Research Publications
    Abstract

    A cryopreservation protocol was developed for Lomandra sonderi (Asparagaceae), an endemic plant of southwest Western Australia used for mine site restoration. Thermal analysis of L. sonderi shoot tips using differential scanning calorimetry was used to detect the formation of ice in shoot tips and consequently allowed optimisation of the time of incubation in plant vitrification solution 2 (PVS2), which attempted to minimise phytotoxicity of, and excessive dehydration by, its cryoprotective components. Sugar pretreatments did not improve survival. Use of a loading solution containing 2 M glycerol and 0.4 M sucrose prior to incubation in PVS2 improved survival of control shoot tips. Preconditioning at 20+/-1 degree C day/night alternating temperature with a 16 h photoperiod or at a constant 5 degree C temperature with a 12 h photoperiod both significantly improved both control shoot tip survival and post-cryopreservation survival. Shoot tips that recovered from liquid nitrogen immersion were successfully re-established as actively growing in vitro plantlets.

    Related items

    Showing items related by title, author, creator and subject.

    • Cryopreservation of somatic germplasm of selected Australian monocotyledonous taxa (Haemodoraceae).
      Turner, Shane (2001)
      The South West Botanical Province of Western Australia is one of the most floristically rich areas of the world with over, 8,000 species present, the majority of which (70%), are endemic to this region. Coupled with this ...
    • Ex situ conservation of the endangered species Androcalva perlaria (Malvaceae) by micropropagation and cryopreservation
      Whiteley, S.; Bunn, E.; Menon, A.; Mancera, Ricardo; Turner, S. (2016)
      Micropropagation and cryopreservation protocols were developed for the threatened Australian species Androcalva perlaria. Vegetative shoots were brought into culture using a simplified surface sterilisation process with ...
    • Monitoring of oxidative status in three native Australian species during cold acclimation and cryopreservation
      Funnekotter, Bryn; Colville, L.; Kaczmarczyk, A.; Turner, S.; Bunn, E.; Mancera, Ricardo (2017)
      © 2017, Springer-Verlag GmbH Germany. Key message: Three wild species exhibited a significant reduction in antioxidants throughout the cryopreservation protocol, whilst the half-cell reduction potential became more oxidised. ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.