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    Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia

    Access Status
    Fulltext not available
    Authors
    Ghassemifar, Reza
    Forster, L.
    Finlayson, J.
    Calogero, T.
    Augustson, B.
    Joske, D.
    Cull, G.
    Date
    2012
    Type
    Journal Article
    
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    Citation
    Ghassemifar, R. and Forster, L. and Finlayson, J. and Calogero, T. and Augustson, B. and Joske, D. and Cull, G. 2012. Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia. Pathology. 44 (7): pp. 632-637.
    Source Title
    Pathology
    DOI
    10.1097/PAT.0b013e32835a0142
    ISSN
    0031-3025
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/11531
    Collection
    • Curtin Research Publications
    Abstract

    Aim: To examine the relative gene expression levels of the anti-apoptotic Bcl-2a and ß isoforms and the pro-apoptotic Baxa and ß isoforms in patients with chronic lymphocytic leukaemia (CLL) and healthy controls (HC). Methods: Peripheral blood was obtained from 36 patients diagnosed with CLL and 10 HC. CD19+ B-lymphocytes were isolated using an antibody coupled magnetic bead isolation system; from these cells the total RNA was isolated and purified. The relative levels of gene expression were examined by quantitative real-time polymerase chain reaction (qReTi-PCR) using primers specific for each isoform. Results: Bcl-2a and Baxa are expressed at higher levels than their ß-isoforms in CLL and HC. Bcl-2a, Bcl-2ß and Baxb expression is increased in CLL while Bax-a is expressed at similar levels to HC. The Bcl-2a/Bcl-2ß ratio is similar in CLL and HC. The Bcl-2a/Baxa ratio is increased in CLL when compared with HC. Conclusion: Bcl-2a and Baxa appear to be the dominant antiand pro-apoptotic isoforms in CLL. The Bcl-2a/Baxa ratio is increased in CLLwhile the Bcl-2a/Bcl-2ß ratio is similar to HC.

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