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dc.contributor.authorGhassemifar, Reza
dc.contributor.authorForster, L.
dc.contributor.authorFinlayson, J.
dc.contributor.authorCalogero, T.
dc.contributor.authorAugustson, B.
dc.contributor.authorJoske, D.
dc.contributor.authorCull, G.
dc.date.accessioned2017-01-30T11:25:16Z
dc.date.available2017-01-30T11:25:16Z
dc.date.created2015-10-29T04:09:57Z
dc.date.issued2012
dc.identifier.citationGhassemifar, R. and Forster, L. and Finlayson, J. and Calogero, T. and Augustson, B. and Joske, D. and Cull, G. 2012. Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia. Pathology. 44 (7): pp. 632-637.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/11531
dc.identifier.doi10.1097/PAT.0b013e32835a0142
dc.description.abstract

Aim: To examine the relative gene expression levels of the anti-apoptotic Bcl-2a and ß isoforms and the pro-apoptotic Baxa and ß isoforms in patients with chronic lymphocytic leukaemia (CLL) and healthy controls (HC). Methods: Peripheral blood was obtained from 36 patients diagnosed with CLL and 10 HC. CD19+ B-lymphocytes were isolated using an antibody coupled magnetic bead isolation system; from these cells the total RNA was isolated and purified. The relative levels of gene expression were examined by quantitative real-time polymerase chain reaction (qReTi-PCR) using primers specific for each isoform. Results: Bcl-2a and Baxa are expressed at higher levels than their ß-isoforms in CLL and HC. Bcl-2a, Bcl-2ß and Baxb expression is increased in CLL while Bax-a is expressed at similar levels to HC. The Bcl-2a/Bcl-2ß ratio is similar in CLL and HC. The Bcl-2a/Baxa ratio is increased in CLL when compared with HC. Conclusion: Bcl-2a and Baxa appear to be the dominant antiand pro-apoptotic isoforms in CLL. The Bcl-2a/Baxa ratio is increased in CLLwhile the Bcl-2a/Bcl-2ß ratio is similar to HC.

dc.titleDifferential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia
dc.typeJournal Article
dcterms.source.volume44
dcterms.source.number7
dcterms.source.startPage632
dcterms.source.endPage637
dcterms.source.issn0031-3025
dcterms.source.titlePathology
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusFulltext not available


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