Cardiomyocyte apoptosis vs autophagy with prolonged doxorubicin treatment: Comparison with osteosarcoma cells
dc.contributor.author | Tacar, O. | |
dc.contributor.author | Indumathy, S. | |
dc.contributor.author | Tan, M. | |
dc.contributor.author | Baindur-Hudson, S. | |
dc.contributor.author | Friedhuber, A. | |
dc.contributor.author | Dass, Crispin | |
dc.date.accessioned | 2017-01-30T11:27:07Z | |
dc.date.available | 2017-01-30T11:27:07Z | |
dc.date.created | 2015-10-29T04:08:53Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Tacar, O. and Indumathy, S. and Tan, M. and Baindur-Hudson, S. and Friedhuber, A. and Dass, C. 2015. Cardiomyocyte apoptosis vs autophagy with prolonged doxorubicin treatment: Comparison with osteosarcoma cells. Journal of Pharmacy and Pharmacology. 67 (2): pp. 231-243. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/11838 | |
dc.identifier.doi | 10.1111/jphp.12324 | |
dc.description.abstract |
Objective Doxorubicin (Dox) is a frontline chemotherapeutic against osteosarcoma (OS) that is plagued by side effects, particularly in the heart. The specific objective of this article is to investigate whether low-dose Dox treatment had pro-autophagic effects in cardiomyocytes as well as osteosarcoma cells. Methods This study characterises apoptotic (Bax) and autophagic (Beclin-1) biomarker levels in human OS and cardiomyocyte cell lines as well as in various tissues when mice are exposed to low (1-mg/kg, thrice weekly) and high (3-mg/kg thrice weekly) dose Dox for a month. Key findings There was a decrease in Bax and increase in Beclin-1 in cardiac tissue in the high-dose group. Dox decreased Beclin-1 in the skin and liver, with no clear indication in the stomach, small intestine and testis. At low Dox doses of 10 and 100-nm in cardiomyocytes and OS cells, there is a pro-apoptotic effect, with a quicker response in the 100-nm condition, and a slower but steady increase of a pro-apoptotic response at the lower 10-nm dose. However, electron microscopy images revealed changes to human OS cells that resembled autophagy. Human prostate, breast and colorectal cells treated with 10-nm Dox showed ~ 40% reduction in cell viability after 24-h. Conclusion In culture, cells of both cardiomyocytes and OS revealed a predominant pro-apoptotic response at the expense of autophagy, although both seemed to be occurring in vivo. | |
dc.publisher | Blackwell Publishing Ltd | |
dc.title | Cardiomyocyte apoptosis vs autophagy with prolonged doxorubicin treatment: Comparison with osteosarcoma cells | |
dc.type | Journal Article | |
dcterms.source.volume | 67 | |
dcterms.source.number | 2 | |
dcterms.source.startPage | 231 | |
dcterms.source.endPage | 243 | |
dcterms.source.issn | 0022-3573 | |
dcterms.source.title | Journal of Pharmacy and Pharmacology | |
curtin.department | School of Pharmacy | |
curtin.accessStatus | Fulltext not available |
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